purePlex™ DNA Library Prep Kit

PRODUCTS

purePlex™ DNA Library Prep Kit

purePlex™ DNA Library Prep Kit

A benefit to purePlex is that, because of its simplicity, users can quickly and seamlessly incorporate the kit with existing methods for high-throughput pipetting.

Speed, Performance, and Auto-Normalization with Unique Dual Indexes

  1. 2.5-hour workflow for 96 samples, 45 min. hands-on time
  2. Auto-normalization of read counts and insert size over 10-fold input range
  3. Unique dual indices included
purePlex™ DNA Library Prep Kit seqWell

Key Features

  1. Fast, flexible workflow with no requirement for full plate processing
  2. Auto-normalization reduces QC burden, improves data consistency
  3. Early pooling for easier sample handling
  4. Reduced GC bias compared to other transposase-based methods
  5. Significant cost and plastics savings

purePlex™ DNA Library Prep Kit Workflow seqWell

purePlex™ DNA Library Prep Kit Workflow seqWell
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    ExpressPlex™ Library Prep Kit

    PRODUCTS

    ExpressPlex™ Library Prep Kit

    ExpressPlex™ Library Prep Kit

    Designed for quick turnaround of plasmid, amplicon, or synthetic construct sequencing, ExpressPlex* is the fastest high-throughput library preparation kit available (based on total time to prepare 96 – 384 samples).

    1. 90-min workflow with 30-min or less hands-on
    2. Fragmentation, barcoding, and amplification in 1 step
    3. No primers/barcodes to buy
    4. Virtually cross-talk free
    5. Up to 6,144 samples prepped and sequenced in 24 hours
    6. NEW: 384-well ultra-high throughput version available
    *Patents Pending
    ExpressPlex™ Library Prep Kit SeqWell

    Benefits of using ExpressPlex

    ExpressPlex allows you to spend your time on data and results, not pipetting.  There are solutions for low-, medium-, and high-throughput labs.  For ultra-high throughput users, we now offer 384-well versions that will enable you to multiplex up to 6,144 samples in a single run. 

    1. Go from extracted samples to libraries on the sequencer in < 1/2 a day
    2. Sequence more samples for less
    3. Easily automate your protocol
    4. Train any lab tech, minimize chance for error
    5. Choice of 96 or 384-well versions to fit your workflow
    6. Reduce labor while increasing efficiency
    7. Decrease chance for errors via minimal handling steps
    8. Everything included: no complex supply chain management of barcodes and primers

    ExpressPlex 96-Well Workflow

    ExpressPlex-Workflow-1024x682

    ExpressPlex 384-Well Workflow

    ExpressPlex-384-WFG-1-1024x918
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      Tagify

      PRODUCTS

      Tagify

      Enable Gene Editing QC Applications with Tagify™ UMI Reagents

      Methods such as CRISPR-Cas9 can exhibit unpredictability in both off- and on-target applications, but seqWell’s Tagify UMI tagging reagents ensure reproducibility, paving the way for the future of gene editing QC.

      Writing the Future of Gene Editing QC

      While genome editing tools like TALENs and ZFNs have transformed biomedical research, CRISPR-Cas9 stands out as a powerful tool since the early 2010s. The therapeutic potential of gene editing is immense, but to ensure precise edits, further research and the development of robust QC methods are essential. Transposase-based approaches, such as seqWell’s Tagify UMI reagents, offer a promising solution. Integrating Tagify UMIs with methods like UDiTaS™ can help establish QC benchmarks, contributing to the creation of safer and more effective gene editing techniques.

      Ensure Consistency and Scalability

      Georgia Giannoukos, Ph.D., Director of Next Generation Sequencing at Editas Medicine, emphasizes the use of seqWell’s custom Tagify reagent for UDiTaS. The consistent batches yield similar tagmentation profiles and editing results, with the flexibility to scale reactions from 96 to 384 wells. This adaptability has allowed the processing of thousands of reactions over a year, ensuring both consistency and scalability in gene editing applications.

      Tagify
      Gene-Editing-QC1-2048x703 (1)

      Maximize Gene Editing QC with Tagify™ and ExpressPlex™ Library Prep Kit

      By harnessing the synergies of Tagify and the ExpressPlex™ Library Prep Kit, researchers can achieve comprehensive gene editing quality control (QC). Tagify, incorporating UMIs, serves as a potent downstream QC method, enabling precise quantification of gene editing efficiency and fidelity at the on-target molecular level, ensuring accurate assessment post-editing.

      Simultaneously, the ExpressPlex™ Library Prep Kit serves as an innovative upstream QC method, allowing users to verify the DNA sequence of reagents before initiating the gene editing process.

      When utilized in tandem, with ExpressPlex upstream and Tagify downstream, researchers create a robust QC framework, effectively sandwiching the gene editing process between two reliable checkpoints. This approach proves valuable when QC activities span different labs within the same company, promoting a streamlined workflow and reproducible results.

      Explore the synergistic potential of this powerful combination in more detail in our latest blog.

      Ready To Order?
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      SeqWell Portfolio

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      Get a call from your local Decode Science representative to help you find the best fit genomics products for you.

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        plexWell LP 384

        PRODUCTS

        plexWell LP 384

        The plexWell™ LP 384 kit is engineered for low-pass whole genome library preparation and sequencing, utilizing the enhanced plexWell workflow. The kit includes major reagents essential for library preparation, featuring Magwise™ paramagnetic beads (DNA polymerase not included). This kit allows efficient library preparation for more than one 96-well plate of samples and normalizes input DNA over a wide range of 5-25 ng.

        plexWell™ LP 384 (Low-Pass Whole Genome) Highlights:

        • NGS multiplexed library generation kit designed for Illumina® platforms.
        • Features assay-ready 96-well fully-skirted low-profile PCR plates in sets of 4 (4 x 96).
        • Provides 2,304 (96 x 24) unique barcode combinations.
        • Includes 6 sets of 4 pool barcodes (PB Set A, B, C, D, E, or F).

        Recommended Application: Ideal for low-depth whole genome/GBS (Genotyping by Sequencing) coupled with imputation and analysis software for comprehensive genomic studies.

        plexWell™ Library Prep Workflow:

        plexWell™ Library Prep Chemistry:

        plexWell96
        Ready To Order?
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        SeqWell Portfolio

        Have a question?

        Get a call from your local Decode Science representative to help you find the best fit genomics products for you.

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          1300 581 991

          plexWell

          PRODUCTS

          plexWell

          plexWell 96

          The plexWell™ 96 (PW096) kit streamlines NGS multiplexed library generation for Illumina® platforms, housed in an assay-ready 96-well fully-skirted low-profile PCR plate. The kit includes major reagents, featuring Magwise™ paramagnetic beads (DNA polymerase not included), facilitating efficient library preparation for one 96-well plate inputs. Notably, it offers normalization of input DNA over a broad range of 3-30 ng, with a cost-saving volume-based pricing structure that can reduce total lab costs by 30-50%.

          Key Features:

          • Comprehensive kit with Magwise™ beads.
          • Efficient library prep for one 96-well plate.
          • Normalization of input DNA (3-30 ng).
          • Volume-based pricing for cost savings.

          Recommended Applications: Ideal for large-scale full-length viral surveillance, Synthetic Construct Sequencing (Amplicons, Plasmids, BACs, etc.), microbiome screening, microbial whole-genome sequencing, scRNA-seq (single-cell RNA sequencing), and low-depth whole-genome/GBS (genotyping by sequencing).

          plexwell-lps-384-featured-1
          plexWell 384

          The plexWell™ 384 (PW384) kit is a comprehensive solution for NGS multiplexed library generation on Illumina® platforms. Each kit includes major reagents, featuring Magwise™ paramagnetic beads (DNA polymerase not included), essential for efficient library preparation for more than one 96-well plate of samples. This kit offers normalization of input DNA across a wide range of 3-30 ng, with a cost-saving volume-based pricing structure, reducing total lab costs by 30-50%.

          Key Features:

          • Comprehensive kit with Magwise™ paramagnetic beads.
          • Efficient library prep for more than one 96-well plate.
          • Normalization of input DNA (3-30 ng).
          • Cost-saving volume-based pricing.

          Recommended Applications:

          • Large-scale full-length viral surveillance.
          • Synthetic Construct Sequencing (Amplicons, Plasmids, BACs, etc.).
          • Microbiome screening.
          • Microbial whole-genome sequencing.
          • scRNA-seq (single-cell RNA sequencing).
          • Low-depth whole-genome/GBS (genotyping by sequencing).
          plexwell-lps-384-featured-1

          plexWell™ Library Prep Workflow:

           

          plexWell™ Library Prep Chemistry:

          plexWell96
          Ready To Order?
          Our team can help you in placing the order. Click below to get a quote and fast ordering.
          SeqWell Portfolio

          Have a question?

          Get a call from your local Decode Science representative to help you find the best fit genomics products for you.

            Or give us a call at:

            1300 581 991