Clonal Genes

Gene Fragments

January 16, 2024/in Partners, Synbio Genes, Twist/by Harshita Sharma

PRODUCTS

Gene Fragments

Overview

Twist Bioscience offers Synthetic Gene Fragments as a fast, economical, and efficient solution for building genes in your research. These gene fragments enhance the cloning process by minimizing the need for colony screening, saving valuable time and reducing overall costs associated with cloning and sequencing.

By leveraging Twist’s Synthetic Gene Fragments, researchers can think bigger, design on a grander scale, and accelerate their discoveries. With the advantage of an industry-leading error rate, customizable lengths and yields, and the convenience of no order limits, Twist’s Gene Fragments offer a cost-effective and time-efficient solution for diverse research needs. To learn more or place an order, researchers can explore the product sheet or directly proceed with ordering from Twist Bioscience.

Fast and Economical:

Gene Fragments for assembly and cloning.
Compatible with all downstream cloning methods.
Priced from 13¢ (AUD) per base pair.
Synthesised in as little as 2 business days.

Screen Less, Discover More:

Industry-leading error rate of 1:7500.
Length: 300 bp – 1800 bp.
Yield: 100 ng – 1 µg.
No order limit.

Twist Bioscience’s DNA synthesis technology stands out by outperforming competitors with exceptionally low error rates. In a direct comparison of Gene Fragment products, Twist consistently demonstrated the lowest error rate, as illustrated in the graph comparing Twist and Integrated DNA Technologies, Inc. (IDT). The results reveal that Twist Gene Fragments exhibit greater sequence accuracy compared to eBlocks and gBlocks, boasting an average of 2-fold greater accuracy in sequence fidelity over gBlocks.

Moreover, Twist Gene Fragments consistently yield the highest percentage of perfect clones, offering a significant advantage in terms of time and cost savings for researchers. The graph showcasing a direct comparison of the percentage of sequence perfect clones across various gene lengths and sequences for three different gene products highlights Twist’s superiority. The data, derived from a set of 63 sequences with diverse gene lengths, reflects the broad applicability of Twist’s technology in addressing the varied requirements of real-world synthetic biology applications.

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SynBio Genes Portfolio

Gene Fragments

Clonal Genes

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High Throughput Antibody Production

January 16, 2024/in Partners, Synbio Antibody, Twist/by Harshita Sharma

PRODUCTS

High Throughput Antibody Production

Twist Bioscience introduces High-Throughput Antibody Production, a revolutionary gene-to-protein workflow designed to address bottlenecks in antibody production. This innovative process leverages Twist’s silicon-based DNA synthesis platform, capable of precisely writing thousands of genes in a single run. The result is rapid and high-throughput production of tens to thousands of diverse antibodies, facilitating efficient screening for biophysical and pharmacokinetic properties.

As a bonus, Twist offers downstream characterization and screening services for all high-throughput antibody production orders. This comprehensive service includes affinity ranking, epitope binning, and more, providing researchers with a streamlined solution for antibody discovery.

The key benefits of Twist’s High-Throughput Antibody Production include the creation of sequence-perfect antibodies, all generated in-house using NGS-verified clonal genes. The workflow offers unlimited capacity, enabling simultaneous production at a scale ranging from tens to thousands of antibodies. Additionally, it is fully customizable, allowing users to select custom antibody sequences, vectors, and isotypes according to their specific research needs.

Researchers can seamlessly expand their antibody production pipeline by uploading the desired antibody sequences, allowing Twist to handle the rest of the process. To learn more about ordering antibodies and taking advantage of this cutting-edge technology, visit the ordering tab for detailed information. Twist Bioscience’s High-Throughput Antibody Production offers a game-changing solution for researchers seeking rapid and customizable antibody discovery and screening.

HOW IT WORKS

The High-Throughput Antibody Production process at Twist Bioscience involves several key steps:

Synthesis and Cloning: Twist synthesizes and clones heavy, light, or single-chain variable sequences into Fc-containing vectors.
Transfection: Paired heavy and light chain or single-chain genes are transfected into HEK293 cells for a period of 4 days.
Harvesting: Supernatants containing the secreted antibodies are harvested.
Purification: Antibodies are purified using Protein A/G.
Inspection: Purified antibodies undergo inspection for concentration, size, and purity using A280 and digital CE-SDS.

Regarding the timeline, from sequence submission to delivery, the process takes approximately 20-25 business days for 1 mL orders and 22-35 business days for 8 mL orders. This efficient and streamlined workflow allows researchers to receive their customized antibodies in a relatively short timeframe, making it a valuable solution for high-throughput antibody production needs.

DATA

Twist Bioscience offers a robust and accelerated antibody screening process, as illustrated in the provided figure. Following antibody production, a total of 518 antibodies were synthesized and purified. After rigorous quality control tests, an impressive 482 antibodies passed the size and purity assessments, with 409 antibodies yielding more than 100 μg per antibody.

The robust yield is determined using LabChip for every purified sample. This involves denaturing the sample to obtain both size and purity data. Capillary electrophoresis sodium dodecyl sulfate (CE-SDS) is then run to collect sizing data benchmarked to a ladder. This provides approximate molecular weight (kDa) and a percent purity for both heavy and light chains. The peak at 7 kD represents the dye front in the analysis.

For those interested in additional data on aspects such as affinity and titer levels, Twist Bioscience offers further insights in their downloadable flyer. This comprehensive screening process demonstrates Twist’s commitment to delivering high-quality antibodies with robust yields, making it an efficient and reliable solution for accelerated antibody production and screening needs.

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Synbio Libraries Portfolio

CAR
Library

TCR
Library

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TCR Library

January 16, 2024/in Partners, Synbio Libraries, Twist/by Harshita Sharma

PRODUCTS

TCR Library

Adoptive cell therapy (ACT) has emerged as a highly effective treatment for cancer, particularly for patients with limited treatment options. This innovative approach harnesses the patient’s own immune system to target and attack cancer cells. One form of ACT, known as Engineered TCR therapy, utilizes engineered T cell receptors to specifically target tumor-specific antigens. The process begins with sequencing tumor biopsy samples to identify tumor mutations and analyzing peripheral blood to uncover the TCR repertoire.

TCR repertoire sequencing can be achieved through two main methods: single-cell sequencing and bulk sequencing, each offering unique advantages. Bulk sequencing allows for a broader sampling of the sequence space, but it comes at the cost of losing information about the alpha-beta TCR pairing. On the other hand, single-cell sequencing captures detailed information about alpha-beta chain pairing and receptor composition, but it has a lower throughput compared to bulk sequencing.

The key benefits of Twist’s technology in this context include high diversity and quality in TCR libraries. Combinatorial assemblies can encompass up to 10,000 gene fragment combinations, ensuring a rich and varied representation. NGS-verified libraries guarantee that over 90% of possible variants are present within 10 times of the mean, ensuring reliability and accuracy. The platform also offers customization, allowing users to define combinatorial variants across alpha and beta chains in TCR Libraries.

Twist’s technology is flexible, accommodating sequences up to 1.5 kb in length, and it is designed at scale with diversity across multiple elements of the sequence. This flexibility, combined with high diversity and quality, positions Twist as a valuable partner in advancing Engineered TCR therapy, providing researchers with the tools needed for comprehensive TCR library customization and optimization.

The process of discovering T Cell Receptors (TCRs) involves screening immune cell repertoires and resynthesizing numerous potential binders. TCR Libraries play a pivotal role in this discovery process, enabling the rapid generation of combinations of TCR beta and alpha chains for high-throughput screening.

Two main approaches are employed: Combinatorial TCR Libraries and Paired Pool TCR Libraries. In Combinatorial TCR Libraries (Figure 1a), the alpha and beta chains are shuffled together, creating a library with greater diversity that extends beyond the identified repertoire. On the other hand, Paired Pool TCR Libraries (Figure 1b) preserve the explicit alpha and beta chain pairing identified through sequencing.

The T Cell Receptor (TCR) Discovery workflow involves partnering with Twist Bioscience to identify and develop novel advanced cell therapies using large-scale, highly diverse TCR libraries. Twist provides highly uniform screening libraries, precise user-defined combinations of gene fragments that allow for efficient and comprehensive screening of desired combinations. This partnership facilitates the discovery of novel TCRs, advancing the development of innovative and effective cell therapies for various applications.

Combinatorial TCR Libraries

Paired Pool TCR Libraries

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Synbio Libraries Portfolio

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CAR Library

January 16, 2024/in Partners, Synbio Libraries, Twist/by Harshita Sharma

PRODUCTS

CAR Library

In recent years, chimeric antigen receptor (CAR) T-cell therapy has emerged as a promising form of immunotherapy, offering new avenues for cancer treatment. Advances in gene transfer technology and gene editing, coupled with innovative CAR designs, have propelled the development of novel cancer therapies. However, despite these strides, several challenges remain, necessitating further research and development efforts to accelerate progress in the field.

CARs typically comprise an extracellular binding domain, a hinge region, a transmembrane domain, and one or more intracellular domains. Fine-tuning each component of the CAR is crucial for enhancing T cell specificity, antigen recognition, and overall T cell function. Studies have demonstrated that even minor modifications to the CAR can significantly impact therapeutic outcomes. Therefore, it is imperative to have a comprehensive library for thorough testing, considering the complexity of each CAR construct.

Twist has pioneered a groundbreaking technology for constructing CAR libraries, allowing seamless shuffling of variants within each domain through a scarless assembly process. The key benefits of this technology include high diversity and quality, with each combinatorial assembly accommodating up to 10,000 gene fragment combinations. NGS-verified libraries ensure that over 90% of possible variants are present within 10 times of the mean. The flexibility and customization options offered by Twist’s technology allow users to design TCR and CAR libraries with user-defined combinatorial variants across specific elements. Additionally, the platform provides flexibility in throughput, enabling the insertion of sequences up to 1.5 kb in length and designing libraries at scale with diversity across multiple elements of the sequence. Twist’s technology represents a significant advancement in the development and optimization of CAR T-cell therapies.

The process begins with synthesizing genes representing different sequence variants for each domain of the Chimeric Antigen Receptor (CAR). These domains, including the extracellular binding domain, hinge region, transmembrane domain, and intracellular domains, can consist of multiple sequence variations. Twist then combines these synthesized genes through a scarless assembly process. This unique assembly method ensures that the combination of these genes results in a highly diverse library for scaffold optimization.

The scarless assembly process enables the seamless merging of the gene variants, creating a comprehensive library that spans a wide range of potential combinations. This technology allows for the exploration of unique domain combinations, unveiling novel functionalities within the CAR scaffold.

The workflow for Chimeric Antigen Receptor (CAR) discovery and optimization involves studying how each domain of the CAR scaffold independently and synergistically influences its functionality. CAR Libraries, generated through this technology, act as a valuable tool for fine-tuning each module. This process helps uncover codependencies among the domains and provides a deeper understanding of their impact on T-cell specificity, antigen recognition, and overall T-cell function.

Researchers can partner with Twist at any stage of their discovery workflow, leveraging both in vivo and in vitro workflows for binder discovery and optimization. Additionally, the use of synthetic libraries from Twist enhances scaffold optimization and validation, providing a versatile and efficient approach to advancing CAR T-cell therapy research and development.

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Synbio Libraries Portfolio

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Spread Out Low Diversity Library

January 10, 2024/in Partners, Synbio Libraries, Twist/by Harshita Sharma

PRODUCTS

Spread Out Low Diversity Libraries

Spread-Out Low Diversity (SOLD) Libraries

Introducing the Spread-Out Low Diversity (SOLD) libraries – the latest tool revolutionizing the mapping of protein sequences and exploring the intricate relationship between proteins and their environment. Designed for researchers seeking efficiency, precision, and cost-effectiveness, SOLD Libraries offer a streamlined approach to investigating combinatorial possibilities.

These libraries stand out with their unique features, providing greater flexibility compared to traditional methods. Suitable for sites with scattered diversity, SOLD Libraries require no template, offering a novel and efficient solution. The precision of SOLD Libraries is unparalleled, ensuring no premature stop codons or unwanted codons, along with precise control over amino acid and codon distribution. This precision surpasses traditional methods such as NNK, TRIM, and epPCR.

Ensuring superior quality, all SOLD Libraries undergo NGS verification of modified regions, rigorous quality control, and verification of all variants. Created using Twist’s patented silicon-based synthesis platform, SOLD Libraries guarantee low error rates, making them cloning-ready and a reliable tool for researchers exploring the variant space. SOLD Libraries provide the ultimate combination of flexibility, precision, and quality for an advanced and efficient protein sequence mapping experience.

Twist’s SOLD Libraries stand out as high-fidelity synthetic constructs, consistently showcasing the capability to closely align amino acid distributions with the requested frequencies. The observed variants in SOLD Libraries reveal a uniform distribution with no dropouts, exemplifying the reliability and precision of this synthetic tool.

A distinctive advantage of Twist’s SOLD Libraries lies in their ability to precisely integrate diversity across a wild-type sequence without constraining it to small variant domains. This unique feature enables researchers to simultaneously explore multiple amino acid positions along a sequence, facilitating a rapid and effective investigation and optimization of a protein’s activity. Twist’s SOLD Libraries, with their scattered diversity along the wild-type sequence, offer a powerful and versatile solution for researchers seeking high-fidelity synthetic constructs in their protein sequence studies.

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Synbio Libraries Portfolio

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Combinatorial Variant Library

January 10, 2024/in Partners, Synbio Libraries, Twist/by Harshita Sharma

PRODUCTS

Combinatorial Variant Libraries

Focused Screening with Precision Variant Technology

Twist’s Precision Variant Library Technology stands out as a groundbreaking approach for focused screening in protein engineering. Powered by Twist’s massively parallel silicon-based DNA synthesis platform, this technology delivers highly uniform and accurate oligos, with an impressive 90% of oligos represented within <2.5x of the mean. Additionally, it boasts an industry-leading low error rate of 1:2,000 nt.

The combination of this cutting-edge synthesis platform with Twist’s well-established molecular biology expertise allows the creation of highly diverse gene mutant libraries. These libraries exhibit excellent variant representation and offer highly specific user-defined compositions, free from unwanted bias or motifs. With precise control over codon usage, amino acid distribution, and length variation, Twist’s library technology enables comprehensive exploration of the variant sequence space. The key benefits include high diversity precision, verified quality through rigorous quality control and NGS verification, and unmatched flexibility in designing sequences, domains, and combinations. This technology outperforms alternatives like TRIM and the degenerate approach, providing researchers with a superior tool for efficient and tailored protein engineering studies.

Twist Bioscience’s silicon-based DNA synthesis platform and library technology redefine the landscape of variant libraries, providing scientists with high-quality data in a more efficient manner. In comparison to competing technologies, Twist’s libraries exhibit less than 1% deviation from the designed amino acid frequency, showcasing exceptional precision. The in-silico DNA synthesis platform seamlessly incorporates binding motifs and length variation across multi-domain libraries, empowering scientists to design and customize variant libraries for a comprehensive analysis of the variant space.

Twist libraries address challenges associated with NNK and TRIM libraries by printing variants base-by-base and screening before synthesis, eliminating stop codons, liability motifs, unwanted mutations, and biases early in the process. This approach enriches the library for requested functional variants, reducing the screening burden. Twist’s industry-leading, ready-to-use, highly-diverse, and precisely designed libraries offer scientists more opportunities to achieve their research goals.

The superiority of Twist’s libraries is further emphasized through a comparison with NNK and TRIM technologies. Twist’s molecular biology expertise and single-base control approach result in high-diversity libraries without confounding motifs. The quality is exemplified in a CVL distribution example, where expected variants are present at user-defined ratios. Additionally, Twist’s Precise Variant Libraries allow users to choose unique CDR sequences, which, when combined with machine learning analysis, can be seamlessly incorporated into fully synthetic libraries for refined exploration of the variant space. Twist’s platform ensures uniform synthesis, minimizing bias and dropouts in downstream workflows. Libraries are QC’d with NGS, enabling the identification and removal of mutations that do not yield improved functions in subsequent iterations of library design.

User-Defined CDR Libraries

Cloning Option For Libraries

Libraries QC’d with NGS

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Synbio Libraries Portfolio

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Site Saturation Variant Library

January 10, 2024/in Partners, Synbio Libraries, Twist/by Harshita Sharma

PRODUCTS

Site Saturation Variant Libraries

Generate 99% of Desired Variants

The utilization of single-site variant libraries is proving invaluable for researchers aiming to delve into a protein’s sequence space and understand the intricate relationship between sequence, protein structure, and function. This innovative approach allows for a comprehensive exploration of variants, providing crucial insights into the molecular dynamics of proteins.

The construction of Twist Site Saturation Variant Libraries takes protein engineering to the next level. Leveraging advanced massively parallel oligonucleotide synthesis through Twist’s proprietary silicon-based DNA synthesis platform, these libraries ensure a precise and controlled crafting of variants. With complete mastery over codon usage, high uniformity at each site, and rigorous quality control verified through next-generation sequencing (NGS), these libraries offer researchers the flexibility to conduct experiments with ease. Whether opting for one position per well in a 96-well plate or pooling all positions in a single tube, this methodology enables the screening of 1 to 20 different amino acids at each position, further enhancing the adaptability and efficiency of protein engineering studies.

Site Saturation Libraries, particularly those constructed by Twist, revolutionize the exploration of sequence space in protein engineering by eliminating codon bias and preventing unwanted substitutions. In contrast to conventional methods like degenerate and NNK approaches, Twist Site Saturation Variant Libraries (SSVLs) offer a superior solution. Traditional methods, such as error-prone PCR and degenerate approaches, often suffer from poor repetitive yield, resulting in less than 50% full-length product in typical libraries. In comparison, Twist libraries yield more usable variants, significantly increasing the effective library size. The figure depicting the observed distribution of amino acids across 65 positions in a protein illustrates the efficiency of Twist SSVLs in maintaining the expected ratios of variants, showcasing their ability to provide highly uniform variant libraries.

Comparing key features, Twist SSVLs outshine error-prone PCR and degenerate approaches on various fronts. They eliminate sequence bias, utilize all 64 codons, prevent undesirable motifs, allow codon optimization, and avoid stop codons. This comprehensive set of advantages positions Twist SSVLs as a powerful tool for efficient sampling of a protein’s sequence space in screening assays, demonstrating their superiority in precision, reliability, and overall effectiveness in protein engineering studies.

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Oligo Pools

January 10, 2024/in Partners, Synbio Oligo Pools, Twist/by Harshita Sharma

PRODUCTS

Oligo Pools

Twist Oligo Pools represent collections of single-stranded oligonucleotides synthesized using state-of-the-art silicon-based DNA writing technology. The innovative synthesis platform employed by Twist Bioscience allows for the simultaneous production of large quantities of high-quality and accurate oligos in each run. This capability facilitates the creation of complex and diverse oligo pools, specifically advantageous for applications such as CRISPR screening.

The process involves users submitting their oligo sequences to Twist Bioscience, which then synthesizes user-designed oligo pool libraries. This service enables researchers to dedicate their time to experimental work and discovery, with the synthesis handled by Twist.

The quality of Twist oligos is underscored by highly uniform synthesis, as evidenced by Next-Generation Sequencing (NGS) quality control data. A representative oligo pool containing 23,000 90mer oligos demonstrates remarkable uniformity, with the corresponding table indicating the metrics for this pool. Twist oligos are synthesized with minimal bias, ensuring high uniformity and complete representation of oligos in the pool.

Furthermore, sequencing analysis of Oligo Pools generated by Twist Bioscience is compared with those from an array-based competitor. The results highlight the superiority of Twist Oligo Pools, as they consistently contain 100% of the expected sequences and exhibit a higher percentage of correct sequences when compared to the competitor’s pool. This emphasis on accuracy and completeness in sequence representation makes Twist Oligo Pools a robust and reliable choice for various molecular biology applications.

New Cloned Oligo Pools

New Cloned Oligo Pools from Twist Bioscience represent a cutting-edge solution aimed at ensuring the foundational quality of oligo pools for successful experiments. Recognizing that errors during synthesis or cloning can adversely impact oligo pool quality by causing over and underrepresentation of desired sequences, Twist now provides an optimized cloning service. This service streamlines the experimental process by alleviating the need for researchers to navigate challenges such as testing PCR amplification conditions, selecting appropriate polymerases and primer pairs, and designing a cloning workflow.

The process involves just two steps. Users submit their oligo sequences to Twist Bioscience, and the company takes care of synthesizing, amplifying, and cloning the user-designed oligo pool libraries. This approach allows researchers to dedicate their time to experimentation and discovery while outsourcing the intricacies of oligo pool synthesis and cloning to Twist Bioscience.

Twist Bioscience emphasizes the high quality of its cloned oligo pools across all lengths, showcasing performance data for pools under 150 nucleotides and those up to 300 nucleotides in length, even with high GC content. The data highlight the uniformity achieved in both cases.

Twist’s cloned oligo pools not only exhibit low error rates and high uniformity but also boast low chimera rates. Chimeras, unwanted hybrid molecules resulting from suboptimal PCR conditions, can lead to improper amplification and recombination of different oligos within the pool. Twist Bioscience provides an illustration comparing an on-target cloned pool without chimeras to an off-target one containing chimeras, emphasizing the importance of avoiding undesired sequences.

The company asserts that its cloned oligo pools, free from chimeras, guarantee the highest quality and accuracy in experimental data. Comparative data between traditional amplification and Twist’s in-house amplification method further supports the efficacy of Twist’s approach in ensuring the integrity of oligo pools for diverse molecular biology applications.

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Other Panels

January 3, 2024/in NGS, Partners, Twist/by Harshita Sharma

PRODUCTS

Other Panels

Mitochondrial Panel

The mitochondrial genome encodes factors critical to energy production, which directly affects the energy state of the cell, tissue, and human individual. Pathogenic mutations of Mitochondrial DNA (mtDNA) are often implicated in a group of complex human diseases which can be difficult to diagnose such as metabolic & neurologic disorders as well as cancer. Interrogation of the human mitochondrial genome by targeted Next Generation Sequencing (NGS) can help investigators shed light on the genetic mechanism behind mitochondrial disorders.

The Twist Mitochondrial Panel is a fixed content panel designed to cover all 16,659 base pairs (bp) and 37 genes of the mitochondrial genome. This panel can be used as a standalone panel or as a spike-in with Twist’s Human Core Exome, Comprehensive Exome as well as Twist’s Custom Panels. Providing industry-leading coverage, uniformity, and flexibility, the Twist Mitochondrial Panel can help investigators enrich for, sequence, and analyze mtDNA variants contributing to disease including incidences of heteroplasmy.

Twist Alliance Diversity SNP Panel

Historical genotyping tools have long grappled with biases toward a limited number of ethnicities, resulting in underrepresentation of significant portions of the global population. Traditional platforms, like microarrays, exacerbate this issue by offering fixed content updated only periodically. To address these inefficiencies and promote equity, researchers can turn to target capture-based Next-Generation Sequencing (NGS) workflows with probe panels designed for diversity and flexibility.

Twist’s expanding Targeted Genomic Block Sequencing (GBS) portfolio includes the Twist Diversity SNP Panel, offering a flexible, ethnicity-neutral gold standard for genotyping. This panel can be used independently or as a spike-in with the Twist Human Comprehensive Exome panel. Its customizable design allows for additional probes to cover specific regions of interest, all synthesized on Twist’s DNA synthesis platform.

Developed in collaboration with the Regeneron Genetics Center, the Twist Diversity SNP Panel seamlessly integrates into existing fully automated exome processing workflows, providing base calls and imputed variants. Genotype imputations can be calculated using open-source tools, with an imputation guide available from Twist Customer Support for those starting with imputation analysis tools.

Note: While the Diversity SNP panel content has not been fully validated through the complete Twist workflow, users are encouraged to contact Twist Customer Support for recommendations on assay set-up.

Twist Alliance Pan-cancer Methylation Panel - 1.5 MB

Methylation sequencing plays a crucial role in detecting and tracing the origins of cancer cells, offering sensitivity in unraveling complex methylation patterns. The Twist Alliance Pan-cancer Methylation Panel – 1.5 MB, a collaborative effort between Twist and AnchorDx, is tailored to focus on cancer-specific methylation patterns across 31 different cancers. This custom target enrichment panel provides deep coverage of clinically relevant targets, enabling the study of methylation patterns crucial for early cancer detection and diagnosis, especially from tumor and liquid biopsy samples.

Constructed with Twist’s state-of-the-art oligonucleotide synthesis platform, the panel boasts high on-target rates and uniformity. It has undergone validation with the Twist Methylation workflow, utilizing liquid biopsy samples from breast cancer, colorectal cancer, and non-small cell lung cancer. The panel design, based on the TCGA database, covers 31 cancer types and 47 disease entities, featuring 13,090 probes targeting approximately 126k CpG sites. Notably, around 74% of the 12k differentially methylated regions (DMRs) overlap with CpG islands, showcasing the panel’s comprehensive coverage.

It’s essential to note that the Twist Alliance Pan-cancer Methylation Panel – 1.5 MB does not carry ISO-13485 certification.

Twist Alliance CNTG Exome - 41 MB

The Twist Alliance CNTG Exome – 41 MB, developed through collaboration between CENTOGENE and Twist, serves as a powerful tool for addressing the challenges associated with the diagnosis of rare diseases. Rare diseases often pose difficulties for physicians, leading patients on prolonged diagnostic journeys. DNA sequencing panels, such as this collaboration, offer comprehensive solutions, providing answers and resolutions for patients undergoing these diagnostic odysseys.

As a leader in rare disease diagnostics, CENTOGENE leverages its expertise in conjunction with Twist’s advanced omics technology to design target enrichment capture panels. The Twist Alliance CNTG Exome – 41 MB ensures highly uniform coverage of the entire exome, including full coverage of the mitochondrial genome. This panel significantly enhances diagnostic yield compared to standard whole exome sequencing, addressing a broad spectrum of disorders encompassing over 7,000 rare diseases.

The panel comprises a total of 483,000 probes, with components such as the Twist Human Comprehensive Exome (396.1K probes), Twist Mitochondrial Panel (139 probes), Twist Alliance Centogene Rare Disease Panel (78.8K probes), and Centogene Spike-In (7.8K probes). Notably, the Twist Alliance CNTG Exome – 41 MB does not carry ISO-13485 certification, emphasizing its designation for research use only and not for diagnostic procedures.

Twist Alliance CNTG Rare Disease Panel - 7.6 MB

The Twist Alliance CNTG Rare Disease Panel, designated for research use only and not for diagnostic procedures, focuses on providing a more streamlined solution for researchers targeting rare diseases. With over 2,500 relevant genes associated with rare diseases, this panel ensures comprehensive coverage, encompassing ≥99.5% of targeted regions within the CNTG Exome footprint.

Containing 78.8K probes, the Twist Alliance CNTG Rare Disease Panel is designed to detect genes linked to various conditions, including rare treatable diseases, early onset childhood disorders, cardiac disorders, neurodegenerative diseases, skeletal abnormalities, and metabolic disorders. This lightweight tool offers researchers the flexibility to capture crucial rare disease coverage without the full exome footprint.

It’s important to note that the Twist Alliance CNTG Rare Disease Panel-7.6MB does not carry ISO-13485 certification, emphasizing its specific designation for research purposes.

Twist Alliance CNTG Hereditary Oncology Panel - 0.2 MB

The Twist Alliance CNTG Hereditary Oncology Panel, expressly designed for research use only and not intended for diagnostic procedures, focuses on providing a targeted solution for identifying genetic predisposition to hereditary tumors. This panel includes 72 carefully selected cancer-associated genes and covers ≥99.5% of targeted regions.

With applications ranging from identifying genetic predispositions in breast cancer to gastrointestinal tumors, Li-Fraumeni syndrome, MEN1, MEN2, paragangliomas, and more, the Twist Alliance CNTG Hereditary Oncology Panel offers a specialized tool for researchers delving into the genetic underpinnings of hereditary cancers.

It’s essential to note that the Twist Alliance CNTG Hereditary Oncology Panel- 0.2MB does not carry ISO-13485 certification and is exclusively designated for research purposes.

Twist Human Ancient DNA Panel

The Ancient Human DNA Panel, a collaborative effort with David Reich’s lab at Harvard University, is designed for studying genetic variation in ancient DNA specimens, typically obtained from bones and teeth. Targeting 1.35 million SNPs, this panel includes a subset covering all 1.23 million polymorphic sites in the human genome published in Fu et al. Nature 2015. This ensures compatibility with published data on numerous ancient individuals, fostering broad comparability.

Developed using Twist’s double-stranded capture probes and hybridization capture reagents, the panel demonstrates high genome coverage and reduced allelic bias. This optimization, as highlighted in Rohland, Mallick et al. Genome Research 2022, enables genome-scale data at significantly reduced sequencing costs compared to shotgun sequencing, thanks to the highly efficient enrichment of human DNA fragments.

It’s important to note that the Twist Alliance Ancient Human DNA Panel is not ISO-13485 certified, emphasizing its exclusive use for research purposes.

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NGS Portfolio

Custom Panels

Viral Panels

Methylation Detection

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