ExpressPlex™ Library Prep Kit

PRODUCTS

ExpressPlex™ Library Prep Kit

ExpressPlex™ Library Prep Kit

Designed for quick turnaround of plasmid, amplicon, or synthetic construct sequencing, ExpressPlex* is the fastest high-throughput library preparation kit available (based on total time to prepare 96 – 384 samples).

  1. 90-min workflow with 30-min or less hands-on
  2. Fragmentation, barcoding, and amplification in 1 step
  3. No primers/barcodes to buy
  4. Virtually cross-talk free
  5. Up to 6,144 samples prepped and sequenced in 24 hours
  6. NEW: 384-well ultra-high throughput version available
*Patents Pending
ExpressPlex™ Library Prep Kit SeqWell

Benefits of using ExpressPlex

ExpressPlex allows you to spend your time on data and results, not pipetting.  There are solutions for low-, medium-, and high-throughput labs.  For ultra-high throughput users, we now offer 384-well versions that will enable you to multiplex up to 6,144 samples in a single run. 

  1. Go from extracted samples to libraries on the sequencer in < 1/2 a day
  2. Sequence more samples for less
  3. Easily automate your protocol
  4. Train any lab tech, minimize chance for error
  5. Choice of 96 or 384-well versions to fit your workflow
  6. Reduce labor while increasing efficiency
  7. Decrease chance for errors via minimal handling steps
  8. Everything included: no complex supply chain management of barcodes and primers

ExpressPlex 96-Well Workflow

ExpressPlex-Workflow-1024x682

ExpressPlex 384-Well Workflow

ExpressPlex-384-WFG-1-1024x918
Ready To Order?
Our team can help you in placing the order. Click below to get a quote and fast ordering.
SeqWell Portfolio

Have a question?

Get a call from your local Decode Science representative to help you find the best fit genomics products for you.

    Or give us a call at:

    1300 581 991

    Tagify

    PRODUCTS

    Tagify

    Enable Gene Editing QC Applications with Tagify™ UMI Reagents

    Methods such as CRISPR-Cas9 can exhibit unpredictability in both off- and on-target applications, but seqWell’s Tagify UMI tagging reagents ensure reproducibility, paving the way for the future of gene editing QC.

    Writing the Future of Gene Editing QC

    While genome editing tools like TALENs and ZFNs have transformed biomedical research, CRISPR-Cas9 stands out as a powerful tool since the early 2010s. The therapeutic potential of gene editing is immense, but to ensure precise edits, further research and the development of robust QC methods are essential. Transposase-based approaches, such as seqWell’s Tagify UMI reagents, offer a promising solution. Integrating Tagify UMIs with methods like UDiTaS™ can help establish QC benchmarks, contributing to the creation of safer and more effective gene editing techniques.

    Ensure Consistency and Scalability

    Georgia Giannoukos, Ph.D., Director of Next Generation Sequencing at Editas Medicine, emphasizes the use of seqWell’s custom Tagify reagent for UDiTaS. The consistent batches yield similar tagmentation profiles and editing results, with the flexibility to scale reactions from 96 to 384 wells. This adaptability has allowed the processing of thousands of reactions over a year, ensuring both consistency and scalability in gene editing applications.

    Tagify
    Gene-Editing-QC1-2048x703 (1)

    Maximize Gene Editing QC with Tagify™ and ExpressPlex™ Library Prep Kit

    By harnessing the synergies of Tagify and the ExpressPlex™ Library Prep Kit, researchers can achieve comprehensive gene editing quality control (QC). Tagify, incorporating UMIs, serves as a potent downstream QC method, enabling precise quantification of gene editing efficiency and fidelity at the on-target molecular level, ensuring accurate assessment post-editing.

    Simultaneously, the ExpressPlex™ Library Prep Kit serves as an innovative upstream QC method, allowing users to verify the DNA sequence of reagents before initiating the gene editing process.

    When utilized in tandem, with ExpressPlex upstream and Tagify downstream, researchers create a robust QC framework, effectively sandwiching the gene editing process between two reliable checkpoints. This approach proves valuable when QC activities span different labs within the same company, promoting a streamlined workflow and reproducible results.

    Explore the synergistic potential of this powerful combination in more detail in our latest blog.

    Ready To Order?
    Our team can help you in placing the order. Click below to get a quote and fast ordering.
    SeqWell Portfolio

    Have a question?

    Get a call from your local Decode Science representative to help you find the best fit genomics products for you.

      Or give us a call at:

      1300 581 991

      plexWell LP 384

      PRODUCTS

      plexWell LP 384

      The plexWell™ LP 384 kit is engineered for low-pass whole genome library preparation and sequencing, utilizing the enhanced plexWell workflow. The kit includes major reagents essential for library preparation, featuring Magwise™ paramagnetic beads (DNA polymerase not included). This kit allows efficient library preparation for more than one 96-well plate of samples and normalizes input DNA over a wide range of 5-25 ng.

      plexWell™ LP 384 (Low-Pass Whole Genome) Highlights:

      • NGS multiplexed library generation kit designed for Illumina® platforms.
      • Features assay-ready 96-well fully-skirted low-profile PCR plates in sets of 4 (4 x 96).
      • Provides 2,304 (96 x 24) unique barcode combinations.
      • Includes 6 sets of 4 pool barcodes (PB Set A, B, C, D, E, or F).

      Recommended Application: Ideal for low-depth whole genome/GBS (Genotyping by Sequencing) coupled with imputation and analysis software for comprehensive genomic studies.

      plexWell™ Library Prep Workflow:

      plexWell™ Library Prep Chemistry:

      plexWell96
      Ready To Order?
      Our team can help you in placing the order. Click below to get a quote and fast ordering.
      SeqWell Portfolio

      Have a question?

      Get a call from your local Decode Science representative to help you find the best fit genomics products for you.

        Or give us a call at:

        1300 581 991

        plexWell

        PRODUCTS

        plexWell

        plexWell 96

        The plexWell™ 96 (PW096) kit streamlines NGS multiplexed library generation for Illumina® platforms, housed in an assay-ready 96-well fully-skirted low-profile PCR plate. The kit includes major reagents, featuring Magwise™ paramagnetic beads (DNA polymerase not included), facilitating efficient library preparation for one 96-well plate inputs. Notably, it offers normalization of input DNA over a broad range of 3-30 ng, with a cost-saving volume-based pricing structure that can reduce total lab costs by 30-50%.

        Key Features:

        • Comprehensive kit with Magwise™ beads.
        • Efficient library prep for one 96-well plate.
        • Normalization of input DNA (3-30 ng).
        • Volume-based pricing for cost savings.

        Recommended Applications: Ideal for large-scale full-length viral surveillance, Synthetic Construct Sequencing (Amplicons, Plasmids, BACs, etc.), microbiome screening, microbial whole-genome sequencing, scRNA-seq (single-cell RNA sequencing), and low-depth whole-genome/GBS (genotyping by sequencing).

        plexwell-lps-384-featured-1
        plexWell 384

        The plexWell™ 384 (PW384) kit is a comprehensive solution for NGS multiplexed library generation on Illumina® platforms. Each kit includes major reagents, featuring Magwise™ paramagnetic beads (DNA polymerase not included), essential for efficient library preparation for more than one 96-well plate of samples. This kit offers normalization of input DNA across a wide range of 3-30 ng, with a cost-saving volume-based pricing structure, reducing total lab costs by 30-50%.

        Key Features:

        • Comprehensive kit with Magwise™ paramagnetic beads.
        • Efficient library prep for more than one 96-well plate.
        • Normalization of input DNA (3-30 ng).
        • Cost-saving volume-based pricing.

        Recommended Applications:

        • Large-scale full-length viral surveillance.
        • Synthetic Construct Sequencing (Amplicons, Plasmids, BACs, etc.).
        • Microbiome screening.
        • Microbial whole-genome sequencing.
        • scRNA-seq (single-cell RNA sequencing).
        • Low-depth whole-genome/GBS (genotyping by sequencing).
        plexwell-lps-384-featured-1

        plexWell™ Library Prep Workflow:

         

        plexWell™ Library Prep Chemistry:

        plexWell96
        Ready To Order?
        Our team can help you in placing the order. Click below to get a quote and fast ordering.
        SeqWell Portfolio

        Have a question?

        Get a call from your local Decode Science representative to help you find the best fit genomics products for you.

          Or give us a call at:

          1300 581 991

          Clonal Genes

          PRODUCTS

          Clonal Genes

          Overview

          Twist Bioscience’s advanced platform is designed to meet diverse DNA synthesis needs, with the capability to synthesize hundreds of thousands of genes each month. Utilizing a silicon-based platform for DNA synthesis, Twist delivers highly precise, sequence-perfect clonal genes of various lengths and complexities, all verified through Next-Generation Sequencing (NGS). Now, with the introduction of the Express Genes service, Twist offers even faster turnaround times, synthesising NGS-verified Clonal Genes in as few as 5 days.

          Twist Bioscience’s Express Genes service offers researchers a cost-effective, customizable, and scalable solution for obtaining high-quality clonal genes with efficient turnaround times. This service empowers researchers to accelerate their projects and advance their scientific endeavors with confidence.

          Low Cost – High Quality:

          Priced from 18¢ (AUD) per base pair.

          No hidden sub-cloning or DNA complexity fees.

          Delivery in as fast as 12 business days.

          DNA Your Way:

          Customize 0.3 – 5 kb genes cloned into a plasmid of your choice.

          Choose from Twist Catalog Vectors or provide your own.

          Four preparation scales (50ng – 2µg | 2µg – 10µg | 10µg – 100µg | 100µg – 1mg).

          Options for normalization and endotoxin-free available.

          Scalable Synthesis:

          No order limits, providing flexibility.

          Same turnaround time regardless of order size.

          Express Genes Service

          Introducing Twist Bioscience’s Express Genes service*— synthesising NGS-verified, sequence-perfect Clonal Genes with an unprecedented order-to-ship turnaround time of 5-7 business days. Explore our full Clonal Genes offering below and experience the speed and efficiency of Express Genes for your research needs.

          Genes Table (Clonal and Express Gene)

          *Terms and Conditions: Eligible Express Genes ship in 5-7 business days. This time will vary based on complexity and length of the sequence. Orders placed outside of the US will incur additional delivery turnaround time. Turnaround time for Clonal Genes is subject to change based on customizations and complexity. Average turnaround time for Clonal Genes is 10-15 business days. New vector onboarding for both Express Genes and Clonal Genes will add additional time.

          DATA

          The data presented illustrates the high quality and precision of Twist Bioscience’s Clonal Genes, showcasing a graphical representation of the standard Next-Generation Sequencing (NGS) verification performed on each clonal gene. The featured clonal gene in the figure serves as an example of an error-free clone. The read depth is indicated for the entire plasmid, and no Single Nucleotide Polymorphisms (SNPs) or insertions/deletions (indels) were detected, emphasizing the accuracy and reliability of Twist’s clonal gene synthesis.

          This data emphasizes Twist’s commitment to providing researchers with sequence-perfect clonal genes, ensuring high-quality and error-free results for a wide range of applications in the field of genetic research and synthetic biology.

          Clonal Genes
          Ready To Order?
          Our team can help you in placing the order. Click below to get a quote and fast ordering.
          Synbio Genes Portfolio

          Have a question?

          Get a call from your local Decode Science representative to help you find the best fit genomics products for you.

            Or give us a call at:

            1300 581 991

            Gene Fragments

            PRODUCTS

            Gene Fragments

            Overview

            Twist Bioscience offers Synthetic Gene Fragments as a fast, economical, and efficient solution for building genes in your research. These gene fragments enhance the cloning process by minimizing the need for colony screening, saving valuable time and reducing overall costs associated with cloning and sequencing.

            By leveraging Twist’s Synthetic Gene Fragments, researchers can think bigger, design on a grander scale, and accelerate their discoveries. With the advantage of an industry-leading error rate, customizable lengths and yields, and the convenience of no order limits, Twist’s Gene Fragments offer a cost-effective and time-efficient solution for diverse research needs. To learn more or place an order, researchers can explore the product sheet or directly proceed with ordering from Twist Bioscience.

            Fast and Economical:

            Gene Fragments for assembly and cloning.
            Compatible with all downstream cloning methods.
            Priced from 13¢ (AUD) per base pair.
            Synthesised in as little as 2 business days.

            Screen Less, Discover More:

            Industry-leading error rate of 1:7500.
            Length: 300 bp – 1800 bp.
            Yield: 100 ng – 1 µg.
            No order limit.

            Twist Bioscience’s DNA synthesis technology stands out by outperforming competitors with exceptionally low error rates. In a direct comparison of Gene Fragment products, Twist consistently demonstrated the lowest error rate, as illustrated in the graph comparing Twist and Integrated DNA Technologies, Inc. (IDT). The results reveal that Twist Gene Fragments exhibit greater sequence accuracy compared to eBlocks and gBlocks, boasting an average of 2-fold greater accuracy in sequence fidelity over gBlocks.

            Moreover, Twist Gene Fragments consistently yield the highest percentage of perfect clones, offering a significant advantage in terms of time and cost savings for researchers. The graph showcasing a direct comparison of the percentage of sequence perfect clones across various gene lengths and sequences for three different gene products highlights Twist’s superiority. The data, derived from a set of 63 sequences with diverse gene lengths, reflects the broad applicability of Twist’s technology in addressing the varied requirements of real-world synthetic biology applications.

            Gene-Fragments-AverageErrorRate-3
            perfect-clones-bp-length@2x
            Ready To Order?
            Our team can help you in placing the order. Click below to get a quote and fast ordering.
            SynBio Genes Portfolio

            Have a question?

            Get a call from your local Decode Science representative to help you find the best fit genomics products for you.

              Or give us a call at:

              1300 581 991

              High Throughput Antibody Production

              PRODUCTS

              High Throughput Antibody Production

              High Throughput Antibody Production

              Twist Bioscience introduces High-Throughput Antibody Production, a revolutionary gene-to-protein workflow designed to address bottlenecks in antibody production. This innovative process leverages Twist’s silicon-based DNA synthesis platform, capable of precisely writing thousands of genes in a single run. The result is rapid and high-throughput production of tens to thousands of diverse antibodies, facilitating efficient screening for biophysical and pharmacokinetic properties.

              As a bonus, Twist offers downstream characterization and screening services for all high-throughput antibody production orders. This comprehensive service includes affinity ranking, epitope binning, and more, providing researchers with a streamlined solution for antibody discovery.

              The key benefits of Twist’s High-Throughput Antibody Production include the creation of sequence-perfect antibodies, all generated in-house using NGS-verified clonal genes. The workflow offers unlimited capacity, enabling simultaneous production at a scale ranging from tens to thousands of antibodies. Additionally, it is fully customizable, allowing users to select custom antibody sequences, vectors, and isotypes according to their specific research needs.

              Researchers can seamlessly expand their antibody production pipeline by uploading the desired antibody sequences, allowing Twist to handle the rest of the process. To learn more about ordering antibodies and taking advantage of this cutting-edge technology, visit the ordering tab for detailed information. Twist Bioscience’s High-Throughput Antibody Production offers a game-changing solution for researchers seeking rapid and customizable antibody discovery and screening.

              HOW IT WORKS

              The High-Throughput Antibody Production process at Twist Bioscience involves several key steps:

              1. Synthesis and Cloning: Twist synthesizes and clones heavy, light, or single-chain variable sequences into Fc-containing vectors.

              2. Transfection: Paired heavy and light chain or single-chain genes are transfected into HEK293 cells for a period of 4 days.

              3. Harvesting: Supernatants containing the secreted antibodies are harvested.

              4. Purification: Antibodies are purified using Protein A/G.

              5. Inspection: Purified antibodies undergo inspection for concentration, size, and purity using A280 and digital CE-SDS.

              Regarding the timeline, from sequence submission to delivery, the process takes approximately 20-25 business days for 1 mL orders and 22-35 business days for 8 mL orders. This efficient and streamlined workflow allows researchers to receive their customized antibodies in a relatively short timeframe, making it a valuable solution for high-throughput antibody production needs.

              v2-HT-IgG-workflow
              1 mL High-throughput Antibody Production Timeline
              8 mL High-throughput Antibody Production Timeline

              DATA

              Twist Bioscience offers a robust and accelerated antibody screening process, as illustrated in the provided figure. Following antibody production, a total of 518 antibodies were synthesized and purified. After rigorous quality control tests, an impressive 482 antibodies passed the size and purity assessments, with 409 antibodies yielding more than 100 μg per antibody.

              The robust yield is determined using LabChip for every purified sample. This involves denaturing the sample to obtain both size and purity data. Capillary electrophoresis sodium dodecyl sulfate (CE-SDS) is then run to collect sizing data benchmarked to a ladder. This provides approximate molecular weight (kDa) and a percent purity for both heavy and light chains. The peak at 7 kD represents the dye front in the analysis.

              For those interested in additional data on aspects such as affinity and titer levels, Twist Bioscience offers further insights in their downloadable flyer. This comprehensive screening process demonstrates Twist’s commitment to delivering high-quality antibodies with robust yields, making it an efficient and reliable solution for accelerated antibody production and screening needs.

              1 mL Expression antibody yield, n=482
              High-purity-antibody-stocks (1)
              Ready To Order?
              Our team can help you in placing the order. Click below to get a quote and fast ordering.
              Synbio Libraries Portfolio

              Have a question?

              Get a call from your local Decode Science representative to help you find the best fit genomics products for you.

                Or give us a call at:

                1300 581 991

                TCR Library

                PRODUCTS

                TCR Library

                TCR Library

                Adoptive cell therapy (ACT) has emerged as a highly effective treatment for cancer, particularly for patients with limited treatment options. This innovative approach harnesses the patient’s own immune system to target and attack cancer cells. One form of ACT, known as Engineered TCR therapy, utilizes engineered T cell receptors to specifically target tumor-specific antigens. The process begins with sequencing tumor biopsy samples to identify tumor mutations and analyzing peripheral blood to uncover the TCR repertoire.

                TCR repertoire sequencing can be achieved through two main methods: single-cell sequencing and bulk sequencing, each offering unique advantages. Bulk sequencing allows for a broader sampling of the sequence space, but it comes at the cost of losing information about the alpha-beta TCR pairing. On the other hand, single-cell sequencing captures detailed information about alpha-beta chain pairing and receptor composition, but it has a lower throughput compared to bulk sequencing.

                The key benefits of Twist’s technology in this context include high diversity and quality in TCR libraries. Combinatorial assemblies can encompass up to 10,000 gene fragment combinations, ensuring a rich and varied representation. NGS-verified libraries guarantee that over 90% of possible variants are present within 10 times of the mean, ensuring reliability and accuracy. The platform also offers customization, allowing users to define combinatorial variants across alpha and beta chains in TCR Libraries.

                Twist’s technology is flexible, accommodating sequences up to 1.5 kb in length, and it is designed at scale with diversity across multiple elements of the sequence. This flexibility, combined with high diversity and quality, positions Twist as a valuable partner in advancing Engineered TCR therapy, providing researchers with the tools needed for comprehensive TCR library customization and optimization.

                The process of discovering T Cell Receptors (TCRs) involves screening immune cell repertoires and resynthesizing numerous potential binders. TCR Libraries play a pivotal role in this discovery process, enabling the rapid generation of combinations of TCR beta and alpha chains for high-throughput screening.

                Two main approaches are employed: Combinatorial TCR Libraries and Paired Pool TCR Libraries. In Combinatorial TCR Libraries (Figure 1a), the alpha and beta chains are shuffled together, creating a library with greater diversity that extends beyond the identified repertoire. On the other hand, Paired Pool TCR Libraries (Figure 1b) preserve the explicit alpha and beta chain pairing identified through sequencing.

                The T Cell Receptor (TCR) Discovery workflow involves partnering with Twist Bioscience to identify and develop novel advanced cell therapies using large-scale, highly diverse TCR libraries. Twist provides highly uniform screening libraries, precise user-defined combinations of gene fragments that allow for efficient and comprehensive screening of desired combinations. This partnership facilitates the discovery of novel TCRs, advancing the development of innovative and effective cell therapies for various applications.

                figure-TCR Combinatorial Assembly Library With Twist_0
                figure-Chimeric antigen receptor (CAR) workflow using Combinatorial Assembly Libraries

                Combinatorial TCR Libraries

                figure-Combinatorial TCR Library Data (1)_0

                Paired Pool TCR Libraries

                figure-Arrayed TCR Library Data (1)_0
                Ready To Order?
                Our team can help you in placing the order. Click below to get a quote and fast ordering.
                Synbio Libraries Portfolio

                Have a question?

                Get a call from your local Decode Science representative to help you find the best fit genomics products for you.

                  Or give us a call at:

                  1300 581 991

                  CAR Library

                  PRODUCTS

                  CAR Library

                  CAR Library

                  In recent years, chimeric antigen receptor (CAR) T-cell therapy has emerged as a promising form of immunotherapy, offering new avenues for cancer treatment. Advances in gene transfer technology and gene editing, coupled with innovative CAR designs, have propelled the development of novel cancer therapies. However, despite these strides, several challenges remain, necessitating further research and development efforts to accelerate progress in the field.

                  CARs typically comprise an extracellular binding domain, a hinge region, a transmembrane domain, and one or more intracellular domains. Fine-tuning each component of the CAR is crucial for enhancing T cell specificity, antigen recognition, and overall T cell function. Studies have demonstrated that even minor modifications to the CAR can significantly impact therapeutic outcomes. Therefore, it is imperative to have a comprehensive library for thorough testing, considering the complexity of each CAR construct.

                  Twist has pioneered a groundbreaking technology for constructing CAR libraries, allowing seamless shuffling of variants within each domain through a scarless assembly process. The key benefits of this technology include high diversity and quality, with each combinatorial assembly accommodating up to 10,000 gene fragment combinations. NGS-verified libraries ensure that over 90% of possible variants are present within 10 times of the mean. The flexibility and customization options offered by Twist’s technology allow users to design TCR and CAR libraries with user-defined combinatorial variants across specific elements. Additionally, the platform provides flexibility in throughput, enabling the insertion of sequences up to 1.5 kb in length and designing libraries at scale with diversity across multiple elements of the sequence. Twist’s technology represents a significant advancement in the development and optimization of CAR T-cell therapies.

                  The process begins with synthesizing genes representing different sequence variants for each domain of the Chimeric Antigen Receptor (CAR). These domains, including the extracellular binding domain, hinge region, transmembrane domain, and intracellular domains, can consist of multiple sequence variations. Twist then combines these synthesized genes through a scarless assembly process. This unique assembly method ensures that the combination of these genes results in a highly diverse library for scaffold optimization.

                  The scarless assembly process enables the seamless merging of the gene variants, creating a comprehensive library that spans a wide range of potential combinations. This technology allows for the exploration of unique domain combinations, unveiling novel functionalities within the CAR scaffold.

                  The workflow for Chimeric Antigen Receptor (CAR) discovery and optimization involves studying how each domain of the CAR scaffold independently and synergistically influences its functionality. CAR Libraries, generated through this technology, act as a valuable tool for fine-tuning each module. This process helps uncover codependencies among the domains and provides a deeper understanding of their impact on T-cell specificity, antigen recognition, and overall T-cell function.

                  Researchers can partner with Twist at any stage of their discovery workflow, leveraging both in vivo and in vitro workflows for binder discovery and optimization. Additionally, the use of synthetic libraries from Twist enhances scaffold optimization and validation, providing a versatile and efficient approach to advancing CAR T-cell therapy research and development.

                  different-sequence-variants-scaffold-optimization
                  T Cell Receptor (TCR) Discovery workflow using Combinatorial Assembly Libraries
                  Ready To Order?
                  Our team can help you in placing the order. Click below to get a quote and fast ordering.
                  Synbio Libraries Portfolio

                  Have a question?

                  Get a call from your local Decode Science representative to help you find the best fit genomics products for you.

                    Or give us a call at:

                    1300 581 991

                    Spread Out Low Diversity Library

                    PRODUCTS

                    Spread Out Low Diversity Libraries

                    Spread-Out Low Diversity (SOLD) Libraries

                    Introducing the Spread-Out Low Diversity (SOLD) libraries – the latest tool revolutionizing the mapping of protein sequences and exploring the intricate relationship between proteins and their environment. Designed for researchers seeking efficiency, precision, and cost-effectiveness, SOLD Libraries offer a streamlined approach to investigating combinatorial possibilities.

                    These libraries stand out with their unique features, providing greater flexibility compared to traditional methods. Suitable for sites with scattered diversity, SOLD Libraries require no template, offering a novel and efficient solution. The precision of SOLD Libraries is unparalleled, ensuring no premature stop codons or unwanted codons, along with precise control over amino acid and codon distribution. This precision surpasses traditional methods such as NNK, TRIM, and epPCR.

                    Ensuring superior quality, all SOLD Libraries undergo NGS verification of modified regions, rigorous quality control, and verification of all variants. Created using Twist’s patented silicon-based synthesis platform, SOLD Libraries guarantee low error rates, making them cloning-ready and a reliable tool for researchers exploring the variant space. SOLD Libraries provide the ultimate combination of flexibility, precision, and quality for an advanced and efficient protein sequence mapping experience.

                    Spread Out Low Diversity Library Page

                    Twist’s SOLD Libraries stand out as high-fidelity synthetic constructs, consistently showcasing the capability to closely align amino acid distributions with the requested frequencies. The observed variants in SOLD Libraries reveal a uniform distribution with no dropouts, exemplifying the reliability and precision of this synthetic tool.

                    A distinctive advantage of Twist’s SOLD Libraries lies in their ability to precisely integrate diversity across a wild-type sequence without constraining it to small variant domains. This unique feature enables researchers to simultaneously explore multiple amino acid positions along a sequence, facilitating a rapid and effective investigation and optimization of a protein’s activity. Twist’s SOLD Libraries, with their scattered diversity along the wild-type sequence, offer a powerful and versatile solution for researchers seeking high-fidelity synthetic constructs in their protein sequence studies.

                    VariantLibraries-SOLD-Distribution (1)
                    VariantLibraries-SOLD-Diversity
                    Ready To Order?
                    Our team can help you in placing the order. Click below to get a quote and fast ordering.
                    Synbio Libraries Portfolio

                    Have a question?

                    Get a call from your local Decode Science representative to help you find the best fit genomics products for you.

                      Or give us a call at:

                      1300 581 991