Combinatorial Variant Libraries

Site Saturation Variant Library

Site Saturation Variant Library

January 10, 2024/in Partners, Synbio Libraries, Twist/by Harshita Sharma

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Site Saturation Variant Libraries

Generate 99% of Desired Variants

The utilization of single-site variant libraries is proving invaluable for researchers aiming to delve into a protein’s sequence space and understand the intricate relationship between sequence, protein structure, and function. This innovative approach allows for a comprehensive exploration of variants, providing crucial insights into the molecular dynamics of proteins.

The construction of Twist Site Saturation Variant Libraries takes protein engineering to the next level. Leveraging advanced massively parallel oligonucleotide synthesis through Twist’s proprietary silicon-based DNA synthesis platform, these libraries ensure a precise and controlled crafting of variants. With complete mastery over codon usage, high uniformity at each site, and rigorous quality control verified through next-generation sequencing (NGS), these libraries offer researchers the flexibility to conduct experiments with ease. Whether opting for one position per well in a 96-well plate or pooling all positions in a single tube, this methodology enables the screening of 1 to 20 different amino acids at each position, further enhancing the adaptability and efficiency of protein engineering studies.

Site Saturation Libraries, particularly those constructed by Twist, revolutionize the exploration of sequence space in protein engineering by eliminating codon bias and preventing unwanted substitutions. In contrast to conventional methods like degenerate and NNK approaches, Twist Site Saturation Variant Libraries (SSVLs) offer a superior solution. Traditional methods, such as error-prone PCR and degenerate approaches, often suffer from poor repetitive yield, resulting in less than 50% full-length product in typical libraries. In comparison, Twist libraries yield more usable variants, significantly increasing the effective library size. The figure depicting the observed distribution of amino acids across 65 positions in a protein illustrates the efficiency of Twist SSVLs in maintaining the expected ratios of variants, showcasing their ability to provide highly uniform variant libraries.

Comparing key features, Twist SSVLs outshine error-prone PCR and degenerate approaches on various fronts. They eliminate sequence bias, utilize all 64 codons, prevent undesirable motifs, allow codon optimization, and avoid stop codons. This comprehensive set of advantages positions Twist SSVLs as a powerful tool for efficient sampling of a protein’s sequence space in screening assays, demonstrating their superiority in precision, reliability, and overall effectiveness in protein engineering studies.

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Synbio Libraries Portfolio

Combinatorial Variant Library

Spread Out Low Diversity Library

CAR Library

TCR Library

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Oligo Pools

January 10, 2024/in Partners, Synbio Oligo Pools, Twist/by Harshita Sharma

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Oligo Pools

Twist Oligo Pools represent collections of single-stranded oligonucleotides synthesized using state-of-the-art silicon-based DNA writing technology. The innovative synthesis platform employed by Twist Bioscience allows for the simultaneous production of large quantities of high-quality and accurate oligos in each run. This capability facilitates the creation of complex and diverse oligo pools, specifically advantageous for applications such as CRISPR screening.

The process involves users submitting their oligo sequences to Twist Bioscience, which then synthesizes user-designed oligo pool libraries. This service enables researchers to dedicate their time to experimental work and discovery, with the synthesis handled by Twist.

The quality of Twist oligos is underscored by highly uniform synthesis, as evidenced by Next-Generation Sequencing (NGS) quality control data. A representative oligo pool containing 23,000 90mer oligos demonstrates remarkable uniformity, with the corresponding table indicating the metrics for this pool. Twist oligos are synthesized with minimal bias, ensuring high uniformity and complete representation of oligos in the pool.

Furthermore, sequencing analysis of Oligo Pools generated by Twist Bioscience is compared with those from an array-based competitor. The results highlight the superiority of Twist Oligo Pools, as they consistently contain 100% of the expected sequences and exhibit a higher percentage of correct sequences when compared to the competitor’s pool. This emphasis on accuracy and completeness in sequence representation makes Twist Oligo Pools a robust and reliable choice for various molecular biology applications.

New Cloned Oligo Pools

New Cloned Oligo Pools from Twist Bioscience represent a cutting-edge solution aimed at ensuring the foundational quality of oligo pools for successful experiments. Recognizing that errors during synthesis or cloning can adversely impact oligo pool quality by causing over and underrepresentation of desired sequences, Twist now provides an optimized cloning service. This service streamlines the experimental process by alleviating the need for researchers to navigate challenges such as testing PCR amplification conditions, selecting appropriate polymerases and primer pairs, and designing a cloning workflow.

The process involves just two steps. Users submit their oligo sequences to Twist Bioscience, and the company takes care of synthesizing, amplifying, and cloning the user-designed oligo pool libraries. This approach allows researchers to dedicate their time to experimentation and discovery while outsourcing the intricacies of oligo pool synthesis and cloning to Twist Bioscience.

Twist Bioscience emphasizes the high quality of its cloned oligo pools across all lengths, showcasing performance data for pools under 150 nucleotides and those up to 300 nucleotides in length, even with high GC content. The data highlight the uniformity achieved in both cases.

Twist’s cloned oligo pools not only exhibit low error rates and high uniformity but also boast low chimera rates. Chimeras, unwanted hybrid molecules resulting from suboptimal PCR conditions, can lead to improper amplification and recombination of different oligos within the pool. Twist Bioscience provides an illustration comparing an on-target cloned pool without chimeras to an off-target one containing chimeras, emphasizing the importance of avoiding undesired sequences.

The company asserts that its cloned oligo pools, free from chimeras, guarantee the highest quality and accuracy in experimental data. Comparative data between traditional amplification and Twist’s in-house amplification method further supports the efficacy of Twist’s approach in ensuring the integrity of oligo pools for diverse molecular biology applications.

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Other Panels

January 3, 2024/in NGS, Partners, Twist/by Harshita Sharma

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Other Panels

Mitochondrial Panel

The mitochondrial genome encodes factors critical to energy production, which directly affects the energy state of the cell, tissue, and human individual. Pathogenic mutations of Mitochondrial DNA (mtDNA) are often implicated in a group of complex human diseases which can be difficult to diagnose such as metabolic & neurologic disorders as well as cancer. Interrogation of the human mitochondrial genome by targeted Next Generation Sequencing (NGS) can help investigators shed light on the genetic mechanism behind mitochondrial disorders.

The Twist Mitochondrial Panel is a fixed content panel designed to cover all 16,659 base pairs (bp) and 37 genes of the mitochondrial genome. This panel can be used as a standalone panel or as a spike-in with Twist’s Human Core Exome, Comprehensive Exome as well as Twist’s Custom Panels. Providing industry-leading coverage, uniformity, and flexibility, the Twist Mitochondrial Panel can help investigators enrich for, sequence, and analyze mtDNA variants contributing to disease including incidences of heteroplasmy.

Twist Alliance Diversity SNP Panel

Historical genotyping tools have long grappled with biases toward a limited number of ethnicities, resulting in underrepresentation of significant portions of the global population. Traditional platforms, like microarrays, exacerbate this issue by offering fixed content updated only periodically. To address these inefficiencies and promote equity, researchers can turn to target capture-based Next-Generation Sequencing (NGS) workflows with probe panels designed for diversity and flexibility.

Twist’s expanding Targeted Genomic Block Sequencing (GBS) portfolio includes the Twist Diversity SNP Panel, offering a flexible, ethnicity-neutral gold standard for genotyping. This panel can be used independently or as a spike-in with the Twist Human Comprehensive Exome panel. Its customizable design allows for additional probes to cover specific regions of interest, all synthesized on Twist’s DNA synthesis platform.

Developed in collaboration with the Regeneron Genetics Center, the Twist Diversity SNP Panel seamlessly integrates into existing fully automated exome processing workflows, providing base calls and imputed variants. Genotype imputations can be calculated using open-source tools, with an imputation guide available from Twist Customer Support for those starting with imputation analysis tools.

Note: While the Diversity SNP panel content has not been fully validated through the complete Twist workflow, users are encouraged to contact Twist Customer Support for recommendations on assay set-up.

Twist Alliance Pan-cancer Methylation Panel - 1.5 MB

Methylation sequencing plays a crucial role in detecting and tracing the origins of cancer cells, offering sensitivity in unraveling complex methylation patterns. The Twist Alliance Pan-cancer Methylation Panel – 1.5 MB, a collaborative effort between Twist and AnchorDx, is tailored to focus on cancer-specific methylation patterns across 31 different cancers. This custom target enrichment panel provides deep coverage of clinically relevant targets, enabling the study of methylation patterns crucial for early cancer detection and diagnosis, especially from tumor and liquid biopsy samples.

Constructed with Twist’s state-of-the-art oligonucleotide synthesis platform, the panel boasts high on-target rates and uniformity. It has undergone validation with the Twist Methylation workflow, utilizing liquid biopsy samples from breast cancer, colorectal cancer, and non-small cell lung cancer. The panel design, based on the TCGA database, covers 31 cancer types and 47 disease entities, featuring 13,090 probes targeting approximately 126k CpG sites. Notably, around 74% of the 12k differentially methylated regions (DMRs) overlap with CpG islands, showcasing the panel’s comprehensive coverage.

It’s essential to note that the Twist Alliance Pan-cancer Methylation Panel – 1.5 MB does not carry ISO-13485 certification.

Twist Alliance CNTG Exome - 41 MB

The Twist Alliance CNTG Exome – 41 MB, developed through collaboration between CENTOGENE and Twist, serves as a powerful tool for addressing the challenges associated with the diagnosis of rare diseases. Rare diseases often pose difficulties for physicians, leading patients on prolonged diagnostic journeys. DNA sequencing panels, such as this collaboration, offer comprehensive solutions, providing answers and resolutions for patients undergoing these diagnostic odysseys.

As a leader in rare disease diagnostics, CENTOGENE leverages its expertise in conjunction with Twist’s advanced omics technology to design target enrichment capture panels. The Twist Alliance CNTG Exome – 41 MB ensures highly uniform coverage of the entire exome, including full coverage of the mitochondrial genome. This panel significantly enhances diagnostic yield compared to standard whole exome sequencing, addressing a broad spectrum of disorders encompassing over 7,000 rare diseases.

The panel comprises a total of 483,000 probes, with components such as the Twist Human Comprehensive Exome (396.1K probes), Twist Mitochondrial Panel (139 probes), Twist Alliance Centogene Rare Disease Panel (78.8K probes), and Centogene Spike-In (7.8K probes). Notably, the Twist Alliance CNTG Exome – 41 MB does not carry ISO-13485 certification, emphasizing its designation for research use only and not for diagnostic procedures.

Twist Alliance CNTG Rare Disease Panel - 7.6 MB

The Twist Alliance CNTG Rare Disease Panel, designated for research use only and not for diagnostic procedures, focuses on providing a more streamlined solution for researchers targeting rare diseases. With over 2,500 relevant genes associated with rare diseases, this panel ensures comprehensive coverage, encompassing ≥99.5% of targeted regions within the CNTG Exome footprint.

Containing 78.8K probes, the Twist Alliance CNTG Rare Disease Panel is designed to detect genes linked to various conditions, including rare treatable diseases, early onset childhood disorders, cardiac disorders, neurodegenerative diseases, skeletal abnormalities, and metabolic disorders. This lightweight tool offers researchers the flexibility to capture crucial rare disease coverage without the full exome footprint.

It’s important to note that the Twist Alliance CNTG Rare Disease Panel-7.6MB does not carry ISO-13485 certification, emphasizing its specific designation for research purposes.

Twist Alliance CNTG Hereditary Oncology Panel - 0.2 MB

The Twist Alliance CNTG Hereditary Oncology Panel, expressly designed for research use only and not intended for diagnostic procedures, focuses on providing a targeted solution for identifying genetic predisposition to hereditary tumors. This panel includes 72 carefully selected cancer-associated genes and covers ≥99.5% of targeted regions.

With applications ranging from identifying genetic predispositions in breast cancer to gastrointestinal tumors, Li-Fraumeni syndrome, MEN1, MEN2, paragangliomas, and more, the Twist Alliance CNTG Hereditary Oncology Panel offers a specialized tool for researchers delving into the genetic underpinnings of hereditary cancers.

It’s essential to note that the Twist Alliance CNTG Hereditary Oncology Panel- 0.2MB does not carry ISO-13485 certification and is exclusively designated for research purposes.

Twist Human Ancient DNA Panel

The Ancient Human DNA Panel, a collaborative effort with David Reich’s lab at Harvard University, is designed for studying genetic variation in ancient DNA specimens, typically obtained from bones and teeth. Targeting 1.35 million SNPs, this panel includes a subset covering all 1.23 million polymorphic sites in the human genome published in Fu et al. Nature 2015. This ensures compatibility with published data on numerous ancient individuals, fostering broad comparability.

Developed using Twist’s double-stranded capture probes and hybridization capture reagents, the panel demonstrates high genome coverage and reduced allelic bias. This optimization, as highlighted in Rohland, Mallick et al. Genome Research 2022, enables genome-scale data at significantly reduced sequencing costs compared to shotgun sequencing, thanks to the highly efficient enrichment of human DNA fragments.

It’s important to note that the Twist Alliance Ancient Human DNA Panel is not ISO-13485 certified, emphasizing its exclusive use for research purposes.

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cfDNA Pan Cancer Reference standards

Viral Controls

December 20, 2023/in Partners, Synthetic Controls, Twist/by Harshita Sharma

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Synthetic Viral Controls

Trusted Controls for Diverse Applications

In the wake of the global demand for diagnostic tests amid the Coronavirus pandemic, laboratories worldwide seek reliable tools for detecting SARS-CoV-2 and Respiratory viruses across various sample types. Our controls offer a trusted solution to address the expanding need for testing, ensuring accuracy and safety in a wide range of applications.

Ensuring Reproducible, High-Quality Results

Positive controls play a pivotal role in maintaining quality standards for diagnostic assays, spanning from development to day-to-day testing. These controls facilitate the verification and validation of diagnostic tests, supporting both next-generation sequencing (NGS) and reverse transcription polymerase chain reaction (RT-PCR) assays, ensuring reproducibility and reliability.

Safe and Effective Alternatives to “Live Virus”

Concerns regarding safety and security associated with controls based on viral nucleic acids extracted from infected patients or live virus cultures led us to develop synthetic controls through gene synthesis. These alternatives offer a safe and effective solution, allowing broader access to diverse strains while mitigating potential risks.

Flexible Formulations for Varied Needs

Tailor your approach with our flexible control formulations—Standard, Assay Ready, or Encapsulated. Whether in a frozen liquid, dried pellet, or encapsulated dried pellet form, our controls provide options for shipping, storage, and stability, meeting the unique requirements of your applications. Ambient shipping reduces costs and enhances accessibility for researchers globally, making our controls a reliable choice in the dynamic landscape of diagnostic testing.

RESPIRATORY VIRUS CONTROLS

Comprehensive Controls for Respiratory Virus Research

Twist’s Synthetic Respiratory Virus Controls encompass a wide spectrum of RNA and DNA viruses crucial for respiratory disease studies. Aligned with the Twist Respiratory Virus Research Panel (PN 103067), these controls seamlessly integrate into Twist Fixed Panel NGS workflows, as well as RT-PCR or qPCR experiments designed by users.

Navigating the Viral Landscape

Refer to the taxonomic tree in the accompanying figure for a visual representation of the viruses covered by Twist Respiratory Virus Controls. The table provides GenBank IDs, virus types, and lengths for each control, facilitating an in-depth understanding of the viral landscape. Custom synthesis options are also available—connect with your local sales representative for additional details.

Simplify Your Research Journey

For further information or to make a purchase, contact your local sales representative or conveniently “Buy Now.” Twist’s Synthetic Respiratory Virus Controls offer a robust foundation for respiratory virus research, enhancing the precision and reliability of your experiments.

SARS-COV-2 CONTROLS

Elevating Quality Control with Twist Synthetic RNA Controls for SARS-CoV-2

Twist Bioscience offers synthetic RNA controls designed for diverse viral genomes, including SARS-CoV-2. With six non-overlapping 5 kb fragments transcribed into ssRNA, these controls provide over 99.9% genome coverage. Available in standard, Assay Ready, and Encapsulated Formats, they cater to various needs with concentrations tailored for flexibility.

Unique Stability in Encapsulated Controls

Twist’s Encapsulated Controls, sealed in a metal capsule around a desiccated pellet, ensure enhanced stability. With room temperature shipping and a five-year shelf life, these controls provide cost-effective and globally accessible solutions.

Contribution to Multiplex Assay Development

The Twist Assay Ready Synthetic SARS-CoV-2 RNA Control, developed with the US CDC, enables simultaneous testing for influenza A, B, and SARS-CoV-2. Originally designed for authorized laboratories, it is now available to customers outside the CDC.

Best Practices and Regulatory Considerations

For comprehensive guidance on storage and use of controls, refer to provided documents: Twist Assay Ready Synthetic Controls Storage and Use Document and Twist Sars-CoV-2 Encapsulated RNA Control Storage and Handling Guide.

Note: This product is authorized for emergency use by the FDA under an EUA for the detection of SARS-CoV-2 nucleic acid. The emergency use is limited to circumstances justifying the authorization.

MONKEYPOX CONTROLS

Twist Synthetic hMPXV Controls: Precision for Targeted Research

Twist’s Synthetic Human Monkeypox Virus (hMPXV) Controls, representing CB and WA clades, cover over 80% of the viral genome. Validated for custom assays and compatible with CDC-recommended PCR procedures, these controls, in two variants, offer precise tiling of the genome at 900bp intervals. With concentrations at approximately 100,000 copies/uL, they provide accuracy and reproducibility. Contact your local sales representative for more information.

Note: The Twist Synthetic hMPXV Controls are not ISO-13485 certified.

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Synthetic Controls Portfolio

Viral
Controls

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cfDNA Pan Cancer Reference standards

cfDNA Pan-cancer Reference Standards

December 20, 2023/in Partners, Synthetic Controls, Twist/by Harshita Sharma

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Twist cfDNA Pan-cancer Reference Standards

NGS-based liquid biopsy is a rapidly evolving application that requires accurate and precise reference standards. The Twist Pan-cancer Reference Standard is a high-quality, standardized control for use in the research and development of NGS-based liquid biopsy assays. This reference standard is ideal for establishing the analytical limit of detection (LoD) for specific cancer variants and as a control to track the quality of an NGS assay workflow and assess the fidelity of the assay process during research and development of such assays.

Comprehensive Detection of Cancer Variants

458 unique naturally occurring cancer variants

132 clinically relevant variants

Covers 84 different genes involved in cancer

Better Design of Background
DNA

Background DNA derived from human cfDNA samples

DNA size profile and post-sequencing profile mimic native cfDNA

Designed for Precision and Flexibility

Seven individual VAF percentages to choose from

Digital Droplet PCR verification of VAF percentages

Convenient test set of all VAF percentages available

About the Data

Comparison of Twist’s Reference standard and competitors

DNA analysis of the Twist cfDNA Pan-Cancer Reference Standard and competitors reference standards show that Twist more closely mimics native cell-free DNA providing a more true to life test result.

Post Sequencing Profile Comparison

Post sequencing library size distribution comparison between Twist and competitors show how similar Twist mimics native cfDNA samples including the jagged leading peaks.

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Synthetic Controls Portfolio

Viral
Controls

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Custom Panels

December 20, 2023/in NGS, Partners, Twist/by Harshita Sharma

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NGS Custom Panels

Custom Panels

Twist Custom Panels can be designed and built to cover a wide range of panel sizes, target regions, and multiplexing requirements — all with the exceptional and consistent performance you’ve come to expect from Twist NGS solutions.

With our incredibly flexible design capabilities, you can easily expand or enhance content on your existing panel or blend together the content from multiple panels with the Twist Custom Blended Panels option. This unprecedented flexibility saves you time while guaranteeing high-quality performance and analysis.

Consistent Performance Across a Range of Panel Sizes and Target Regions

Design a Twist Custom Panel in minutes using a list of gene names or a target bed file. Regardless of panel size or target region, Twist Custom Panels consistently deliver excellent performance.

Robust Performance During Multiplex Target Enrichment

Twist Custom Panels deliver high-quality performance across 1-, 8-, and even 16-plex enrichment reactions:

High uniformity for all levels of multiplexing
High on-target rates do not vary with higher levels of multiplexing
Low duplication rates across all levels of multiplexing

Verified by NGS to Ensure Reproducibility from Lot to Lot

As demonstrated by NGS-based quality analysis, Twist Custom Panels demonstrate a low lot-to-lot variation. Every Twist Custom Panel is sequenced to ensure consistent quality and performance across all probes within a panel.

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Methylation

December 20, 2023/in NGS, Partners, Twist/by Harshita Sharma

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NGS Methylation Detection

NGS Methylation Detection System

The Twist NGS Methylation Detection System provides a robust, end-to-end sample preparation solution for identifying methylated regions in the human genome. The workflow employs a unique enzymatic process from New England Biolabs® that is much less damaging to DNA, alongside Twist‘s Custom Methylation Panel design.

Whether you are investigating cellular differentiation or screening liquid biopsies for cancer, the system offers the most efficient methylation detection available.

NEBNext® EM-seq™ Kit for Twist Targeted Methylation Sequencing

In partnership with New England Biolabs (NEB), Twist Bioscience offers a new methylation sequencing workflow that improves the quality of libraries and removes the need for damaging bisulfite treatment during prep.

The workflow features enzymatic conversion of unmethylated cytosines (see figure) to identify sites of methyl-cytosine (5mC) and hydroxymethyl-cytosine (5hmC).

Enzymatic conversion produces more intact libraries with better representation, and ultimately achieves more sensitive methylation detection. The library preparation system is suitable for whole genome sequencing and downstream enrichment with Twist Methylation Panels.

EM-seq conversion involves a series of enzymatic steps to convert unmethylated cytosines into uracils. The final result is the same as conventional bisulfite conversion, making EM-seq compatible with existing analysis pipelines that use Bismark and bwa-meth.

Twist Human Methylome Panel

The Twist Human Methylome Panel targets 3.98M CpG sites through 123 Mb of genomic content to target biologically relevant methylation markers. Expansive content makes this panel an ideal choice for investigators to explore the methylation fraction in a diverse range of applications from cancer metastasis, human development, and functional genomics.

The panel is optimized and validated for use with the Twist methylation detection system for a complete end-to-end workflow with industry leading performance. High capture efficiency increases the sensitivity of detection across the footprint of the epigenome while decreasing sequencing costs. The panel is ideal for screening cohort samples and differentially methylated region discovery.

Enhanced Coverage, Advantages, and High Performance: A Comparative Analysis with Microarrays

The Twist Human Methylome Panel offers comprehensive coverage of the genome, targeting 3.98 million CpG sites within 123 Mb of genomic content. It efficiently identifies 84% of CpG islands and covers an additional 105,288,339 bases of related regions. In comparison to average microarrays, the panel overcomes static content limitations and enhances coverage across the epigenome. Microarrays suffer from constraints in methylation detection at extreme ends due to background noise and saturation, whereas the Methylome Panel, utilizing hybrid-capture panels and Next-Generation Sequencing (NGS), provides expanded content, single-base resolution, and a higher dynamic range for more accurate detection of differentially methylated regions. The panel’s performance is notable, achieving 90% coverage at 30x depth, 95% on-target rates, and high uniformity with a fold 80 of 1.54. Its efficient capture metrics instill confidence in accurate methylation fraction detection while minimizing sequencing costs.

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Viral Panels

December 20, 2023/in NGS, Partners, Twist/by Harshita Sharma

PRODUCTS

NGS Viral Panels

Comprehensive Viral Research Panel

The Twist Comprehensive Viral Research Panel is a highly versatile tool designed for comprehensive novel virus detection. It consists of 1,052,421 unique probes derived from reference sequences in databases like RefSeq, FluDB, and VIPR, covering a wide range of viral families affecting humans and animals. This panel can detect novel and evolved viral strains, including those related to specific outbreaks.

The panel demonstrates success in detecting highly divergent viral sequences, such as the spike region of a newly discovered coronavirus and a segment from an H1N1 influenza outbreak. With a mismatch sensitivity defined through various tests, it can capture highly evolved viral sequences with over 99.8% coverage.

Furthermore, the Twist Comprehensive Viral Research Panel allows for the enrichment of novel viruses, showcasing its ability to capture sequences with up to 10% variation. It also supports multiplex detection of diverse viruses, making it suitable for metagenomic applications in various sample types. In a co-infection assay, the panel successfully captured four different virus types spiked into human RNA with high efficiency, demonstrating its multiplexing capabilities.

Respiratory Virus Research Panel

The Twist Respiratory Panel is a powerful tool designed for the detection of common human respiratory pathogens through a single test. It comprises 41,047 probes compiled from the GenBank database, allowing researchers to distinguish COVID-19 symptoms from those of other respiratory illnesses, whether influenza- or non-influenza-related. This panel facilitates high-resolution Next-Generation Sequencing (NGS) by enriching viral sequences, enabling the high-sensitivity detection of viral material, even in challenging samples, with more than 5000x enrichment. Post-enrichment, it achieves coverage of over 99.9% of the genome at 1X or greater.

The panel demonstrates its effectiveness in detecting viral standards from different viral families. Synthetic Viral Controls, when spiked into human carrier RNA, show significant enrichment, with over 70% of reads coming from viral genomes, representing at least a 2500-fold enrichment over the spiked-in content.

Furthermore, the Twist Respiratory Panel supports multiplexing for higher throughput and lower cost. Comparisons using multiplex and single-plex hybridization reactions at different viral titers show that 8-plex capture provides comparable efficient enrichment as a single-plex capture. This is demonstrated using the Twist Synthetic Viral Controls and the Twist Respiratory Virus Research Panel, showcasing the versatility and cost-effectiveness of multiplexing in this respiratory virus detection system.

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Exome Panels

December 20, 2023/in NGS, Partners, Twist/by Harshita Sharma

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Exome Panels

Exome 2.0

Twist Exome 2.0 is designed to detect rare and inherited diseases, as well as germline cancers. This panel’s high uniformity and low off-target rate deliver best-in-class sequencing efficiency, enabling quality data to be collected with less sequencing. With superior coverage of major genetic databases (RefSeq, CCDS, GenCode, Clinvar, ACMG73 and more) and the addition of clinically relevant non-coding pathogenic and likely pathogenic variants, Twist Exome 2.0 provides the value of multiple clinical panels all wrapped into one, easily customizable package.

Twist Alliance VCGS Exome - 40.1 MB

Victorian Clinical Genetics Services (VCGS) and Twist have collaborated to address the challenges physicians face in diagnosing heritable diseases when routine testing falls short. The result is the Twist Alliance VCGS Exome – 40.1 MB, a diagnostic panel drawing on VCGS’s extensive clinical genomics experience. This panel covers the entire exome, providing dedicated boosting of clinically relevant genes (Mendeliome), and is designed with special attention to additional coverage of clinically relevant loci beyond traditional gene coding regions.

The Twist Alliance VCGS Exome – 40.1 MB is a highly uniform panel, considered best-in-class, and aims to facilitate the identification of alleles linked to heritable diseases. Whether used for carrier screening or pre- and post-natal testing, this panel is intended to streamline the diagnostic process.

It’s important to note that the content of the Twist Alliance VCGS Exome – 40.1 MB has not been fully validated through the complete Twist workflow, and for guidance on assay set-up, contacting Twist Customer Support is recommended. Additionally, this panel is not ISO-13485 certified.

Twist Alliance Clinical Research Exome - 34.9 MB

The Twist Alliance Clinical Research Exome, developed in collaboration with the Broad Institute Genomics Platform, is a versatile tool designed for comprehensive exome sequencing. Utilizing validated data from clinical patient samples, this panel is constructed on Twist’s flexible Next-Generation Sequencing (NGS) platform, allowing easy and rapid customization of content. It provides a thorough examination of the exome, with additional enrichment focusing on clinically relevant areas associated with cancer, rare diseases, and inherited conditions. Leveraging the exceptional uniformity of Twist NGS probes, the assay achieves cost and throughput efficiency. The Broad Institute Genomics Platform has already processed over 250,000 samples, showcasing the panel’s effectiveness and keeping the institute at the forefront of exome sequencing. The panel’s design encompasses the Twist Core exome, the mitochondrial genome, and validated coding and non-exonic regions of interest, including ACMG73 genes, supplemental coverage of OMIM and COSMIC regions, and specific targets defined by Broad. It offers a comprehensive solution for a range of research applications. Important to note, the content of the Twist Alliance Clinical Research Exome – 34.9 MB has not been fully validated through the complete Twist workflow. For guidance on assay set-up, users are advised to contact Twist Customer Support. Additionally, this panel is not ISO-13485 certified.

Mouse Exome Panel

The mouse is an extremely important model system for studying genetic variation, tumor mutations, and phenotypic outcomes as well as the therapeutic effect of pharmaceutical agents. As genetic variant databases are continuously updated, the Twist Mouse Exome panel is thoughtfully designed and built from the most current databases. When combined with Twist’s expanding portfolio of library preparation and enrichment reagents, the complete toolset allows researchers to achieve industry-leading coverage across target regions while optimizing sequencing cost and sample throughput.

Twist Alliance Canine Exome - 40.5 MB

Understanding the genetic variations among dog breeds is crucial for unraveling the genetic regulation of traits and understanding the basis and progression of diseases in dogs. Comprehensive gene panels, such as exomes, play a pivotal role in enhancing veterinary diagnostics and associated clinical medicine. These Next-Generation Sequencing (NGS) panels, like the Twist Alliance Canine Exome developed in collaboration with the Broad Institute’s Karlsson lab, are essential tools for advancing canine genomic research. They contribute to improved understanding of canine cancers and potential therapeutics.

The Twist Alliance Canine Exome panel is designed to achieve the following objectives:

Covers coding exons of canine genes.
Facilitates comparative genomic studies between canine and human genomes.
Includes regions of known importance in human cancers.
Allows for cost-effective deep sequencing.

Furthermore, canine genomic research has demonstrated benefits for human medical research, revealing genetic similarities between human and canine tumors, such as Copy Number Variations (CNVs), differential gene expressions, and structural chromosome abnormalities. This interdisciplinary approach underscores the potential for insights gained in canine genetics to contribute to advancements in human medicine.

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