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Jumpcode Genomics

Boost sensitivity in genomics by removing undesired fragments prior to sequencing with DepleteX®

Don’t waste your sequencing reads on abundant and uninformative sequences, which limits the detection of lower abundance signals.

Jumpcode Genomics’ Deplete X technology removes fragments prior to sequencing by harnessing CRISPR-Cas9 to degrade uninformative sequences in next generation sequencing(NGS) libraries.

This can boost detection of low-level gene expression, somatic mutation detection and better pathogen detection.

Bulk RNA-Seq

Abundant ribosomal, mitochondrial, insulin and housekeeping genes

Single Cell Sequencing

Ribosomal, mitochondrial, and non-variable genes

DNA Sequencing

Repetitive DNA, contaminating human DNA in microbial samples

Benefits of DepleteX technology

Highly programmable

Increases sensitivity

Simple automatable workflow

Genomic analysis platform

How DepleteX  Works

DepleteX operates through a process of mediated depletion, seamlessly integrated into Next-Generation Sequencing (NGS) library preparation protocols at strategic points tailored to specific applications. The method involves the formation of CRISPR-Cas9 complexes, utilizing a pool of meticulously designed guide RNAs.

These CRISPR-Cas9 complexes are directed to target sequences of interest within the genomic DNA. Upon binding, Cas9 initiates precise cuts at the targeted sequences, rendering them inaccessible for PCR amplification. Consequently, these targeted sequences are effectively excluded from the resulting sequencing library.

By leveraging DepleteX, researchers can precisely remove undesired sequences from their NGS libraries, allowing for focused analysis of specific genomic regions or elements of interest while minimizing background noise and maximizing sequencing efficiency.

Jumpcode Crispr clean workflow

Let’s Find You an Application That Helps Your Research

Get a call from your local Decode Science representative to help you find the best fit genomics products for you.

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    1300 581 991