The Dovetail™ Hi-C Kit uses a single restriction enzyme (DpnII) for chromatin fragmentation prior to proximity ligation. The kit provides all the reagents necessary for performing the proximity ligation steps on animal, plant and prokaryotic samples prior to NGS library generation. The kit is compatible with the Dovetail™ Library Module for Illumina (Cat # 25004) and 3rd party standard paired-end library preparation kits for Illumina sequencing.
Specifications
| Reactions | 8 |
|---|---|
| Validated Samples | Mammalian cells, Mammalian tissues, nucleated blood, non-nucleated blood, young plant leaves, insects |
| Technique | Hi-C |
| Labelling | Research Use Only |
| Application(s) | Genome Assembly, chromatin conformation analysis, and structural variation detection |
Key Benefits
The Dovetail™ Hi-C Kit offers the following key benefits:
- Proven Hi-C chemistry well accepted in the genome assembly and chromatin conformation research fields
- 6 hour protocol with less than 3 hours hands-on time easily adopted by molecular biology and genomics labs
- Single source kit containing specialized reagents necessary to perform the proximity ligation portion of the protocol (customer provides formaldehyde, EtOH, RNase/DNase free water and SPRIselect beads).
Documents
Manuals & Protocols
Product Resources
Scientific Literature
- The Hitchhiker’s guide to Hi-C analysis: Practical guidelines
- Novel insights into chromosomal conformations in cancer
- Advances in Genomic Profiling and Analysis of 3D Chromatin Structure and Interaction
- Hi-C analysis: from data generation to integration
- Hi–C: A comprehensive technique to capture the conformation of genomes
- HiGlass
Contents & Storage
Module 1 of 2 (Shipped with cold packs; store 2 to 8 degrees Celsius)
- TE Buffer pH 8.0
- Wash Buffer
- TWB Solution
- 2x NTB Solution
- LWB Solution
- NWB Solution
- Chromatin Capture Beads
- Crosslink Reversal Buffer
- Steptavidin Beads
- 10x RBC Lysis Buffer
- 20% SDS
- 40 mM Calcium Chloride