New Features for Clonal Genes
We’ve enhanced our Clonal Genes products with additional plasmid prep sizes and other options including normalization, endotoxin-free DNA, and a variety of tube and plate options to meet your research needs.
Performance at Scale
Our silicon-based, high-precision DNA synthesis platform results in higher quality genes and significantly increased throughput and scalability.
We offer both Clonal Genes and Gene Fragments for your research needs:
Clonal Genes are synthesized DNA cloned into a plasmid vector and NGS sequence verified.
Gene Fragments are linear synthesized DNA sequences for direct cloning or larger gene assembly.
Twist’s Genes offering gives you the flexibility to get the DNA you want, the way you want it. Think bigger, expand your design scope, and accelerate discovery.
Twist Genes in Action
Learn how scientists are using Twist Genes in the Pandemic Protection Platform program, an accelerated antibody development process that aims to provide access to treatments within 60 days.
Clonal Genes
Twist Bioscience’s platform is capable of synthesizing thousands of genes each day to meet all your DNA needs. Our silicon-based scalability and high precision oligo production results in high quality DNA synthesis and assembly; qualities that allow Twist to create perfect genes of various lengths and difficulty, NGS-verified and delivered on-time when you need them.
Benefits
Low Cost – High Quality- No hidden sub-cloning or DNA complexity fees
- From 11 business days
DNA Your Way
- 0.3 – 5 kb genes cloned into a plasmid of your choice
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Choose a Twist Catalog Vector or send us yours
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4 prep scales (50ng – 2µg | 2µg – 10µg | 10µg – 100µg | 100µg – 1mg)
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Normalization and endotoxin free options available
Scalable Synthesis
- No order limits
- Same turnaround regardless of order size
Sequence Perfect Genes
A graphical representation of our standard NGS-verification performed on every clonal gene. The clonal gene in this figure is an example of an error-free clone. The read depth is indicated for the entire plasmid and no SNPs or indels were detected.
Clonal Gene Success Rate
Twist Bioscience’s observed success rate for clonal genes. The success rate is defined as the percentage of clonal genes ordered that were delivered without any SNPs or indels.
Gene Fragments
Twist Bioscience Gene Fragments improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs. Think bigger, design on a grander scale, and accelerate your discoveries.
BENEFITS
Fast and Economical
- 10 to 15 business days from order to delivery
- Gene Fragments for assembly and cloning
- Compatible with many downstream cloning methods
Screen Less, Discover More
- Lowest error rate 1:3000
- 100 ng – 1 µg yield
- 300 bp – 1800 bp gene fragments
- Order as little as one gene fragment
Reduce Costs with High Quality
Comparison of total costs for DNA synthesis, cloning, and sequence verification of constructs built with gene fragments. Sequencing more clones of fragments synthesized by the competitor is required to obtain correct sequence with 99% confidence, increasing the total cost for each gene.
Performance for Less
Twist’s DNA synthesis technology exhibits extremely low error rates, the average error rate is one in 3,000 base pairs. This level of precision makes larger gene assembly more reliable and easier to achieve.
Screen Less
Accurate fragment synthesis accelerates discovery. Twist Bioscience gene fragments enable you to build more constructs and minimize the time and cost of screening for perfect clones.
Clonal Genes Product Sheet
Twist Bioscience is transforming gene synthesis, a process at the core of synthetic and molecular biology. Our silicon-based DNA writing platform significantly increases gene synthesis throughput and scalability, while also reducing turnaround time and price per base.
Gene Fragments Product Sheet
Twist Bioscience gene fragments improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs.
Build a better DNA Assembly line
Arzeda leverages technology from Labcyte, TeselaGen and Twist Bioscience to accelerate their synthetic biology workflow.
Pathway Engineering Through 5 kb Gene Synthesis
A rapid design-build-test cycle is vital to metabolic engineering applications in which researchers re-program microorganisms to produce non-native molecules of interest. To produce these molecules, researchers typically add one gene or an entire pathway of genes into a host organism. These genes encode proteins or enzymes that, when expressed in a microbial host, convert a common substrate into a molecule of interest.
Speeding Up Antibody Discovery for Infectious Disease
Tasked with an ambitious goal from DARPA to develop a rapid response to help medical workers fight viral diseases in the field, Vanderbilt University Medical Center has already reduced the time to develop antibodies significantly. High-throughput, synthetic genes from Twist Bioscience have allowed the lab to expedite this process.
Amyris discusses Next-Generation Platforms for Strain Optimization
Watch this webinar showing how Twist Genes are enabling this innovative company with their microbial engineering efforts. https://www.twistbioscience.com/genes_bioengineeringwebinar_sun
Twist Vectors Product Sheet
Twist Bioscience synthesizes high-quality, NGS-verified custom genes at a… cost and scale that are otherwise unavailable. For researchers wanting to replicate their synthetic genes or use them in expression studies, Twist provides the option of delivery in a diverse selection of cloning and expression vectors through a convenient online ordering platform.
Twist Tips for Genes: How to Design Your Gene
Twist Bioscience uses a silicon-based DNA synthesis platform to generate… high-quality synthetic DNA. Designing and ordering genes from Twist Bioscience is simple when using our online ordering platform. This document provides tips for optimizing and customizing Twist Clonal Genes and Gene Fragments for a variety of applications.
Twist Tips for Genes – Meeting Minimum Length Requirements for Genes in Custom Vectors
When you submit a sequence for synthesis, the scoring algorithm checks the sequence to determine whether it can be synthesized, and one of the first things it checks is whether the sequence meets the minimum length requirement of 300 bp. Use this document to help you design your Genes when they do not meet the minimum length requirement.
Twist DNA Resuspension Guidelines
Twist Bioscience DNA products are dried down and shipped in either 2 mL microcentrifuge tubes or 96- well plates. Although double-stranded DNA and single-stranded oligonucleotides are stable under most standard laboratory storage conditions, it is important to consider the following best practices to maintain the high quality of the DNA synthesized by Twist Bioscience.
From Design to Protein: Cell-Free Protein Expression Using Twist Gene Fragments
A cornerstone of synthetic biology is designing DNA sequences and testing the characteristics and functions of the resultant proteins. Here, Twist Gene Fragments were used as DNA input of a cell-free protein expression study using multiple commercially available in vitro protein production kits. The results demonstrate that Twist Gene Fragments enable high-throughput protein expression and functional testing within a single day.
Gene Fragments Cloning Guidelines
Twist Bioscience’s Gene Fragments are double-stranded DNA, 300 to… 1800 base pairs in length. These high-quality fragments come with unique 5’ and 3’ adapter sequences.
Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer
Machine Learning-Guided Prediction of Antigen-Reactive In Silico Clonotypes Based on Changes in Clonal Abundance through Bio-Panning
Crystal structure of Nsp15 endoribonuclease NendoU from SARS-CoV-2
Continuous evolution of SpCas9 variants compatible with non-G PAMs
Convergent selection in antibody repertoires is revealed by deep learning
De novo protein design enables precise induction of functional antibodies in vivo
In situ readout of DNA barcodes and single base edits facilitated by in vitro transcription
A bottom-up approach for the de novo design of functional proteins