The Dovetail™ Omni-C™ Kit uses a sequence independent endonuclease for chromatin digestion prior to proximity ligation. The Omni-C™ Kit provides all the reagents necessary for performing the proximity ligation steps on plant and animal samples prior to NGS library generation. The kit is compatible with the Dovetail™ Library Module for Illumina (Cat # 25004) and 3rd party standard paired-end library preparation kits for Illumina sequencing.
Specifications
| Reactions | 8 |
|---|---|
| Validated Samples | Plant and Animal cells and tissues |
| Technique | Hi-C |
| Labeling | Research Use Only |
| Application(s) | Genome Assembly, Chromatin conformation analysis, SNP and structural variant detection |
Key Benefits
The Dovetail™ Omni-C™ Kit offers the following key benefits:
- Sequence-independent chromatin fragmentation enables genome-wide detection of chromatin contacts (up to 20% of the genome lacks coverage using restriction enzyme based Hi-C approaches)
- Even genome-wide coverage, similar to shotgun sequencing, enables SNP calling, chromosome phasing, genome assembly, and structural variant detection
- Lower sequencing burden to reach desired depth of coverage saves time and cost
Chromatin Interactions
Omni-C Libraries Enable Genome Wide Resolution of Chromatin Interactions
Due to the use of a sequence-independent endonuclease for chromatin digestion, the Omni-C Kit offers all the characteristics of a Hi-C approach without the sequence bias inherent to restriction enzyme based Hi-C approaches. Compared to restriction enzyme-based Hi-C approaches, Omni-C provides the following benefits:
- Significant overlap with data generated using restriction enzyme based approaches but enriched in long-range cis reads
- Improved resolution when viewing chromatin conformation and looping interactions
- Most complete view of genome-wide chromatin conformation through dramatically increased resolution of topological interactions that occur in regions with low restriction enzyme density
SNPs & Phasing
SNPs & Chromosome Phasing
The even sequence coverage associated with Omni-C libraries enables genome-wide SNP calling and downstream applications based on SNP information. For example:
- Improved coverage of Omni-C libraries enables chromosome phasing with low switch error rates
- Best possible approach for whole-genome physical phasing using Illumina short reads
Large SVs
Large SVs Are Captured In Omni-C Data
The proximity ligation data can be used to detect and confirm chromosomal rearrangements in cancer samples at a high resolution. Using open-source software tools such as HiGlass, contact matrices enable the quick visualization of such large structural variants
Documents
Manuals & Protocols
Mammalian Samples
Non-Mammalian Samples
Safety Data Sheets (SDSs)
Scientific Literature
- The Hitchhiker’s guide to Hi-C analysis: Practical guidelines
- Novel insights into chromosomal conformations in cancer
- Advances in Genomic Profiling and Analysis of 3D Chromatin Structure and Interaction
- Hi-C analysis: from data generation to integration
- Hi–C: A comprehensive technique to capture the conformation of genomes
- Mapping 3D genome architecture through in situ DNase Hi-C
- Using DNase Hi-C techniques to map global and local three-dimensional genome architecture at high resolution
- Bipartite structure of the inactive mouse X chromosome
Contents & Storage
Module 1 of 2 (Shipped with cold packs; store 2 to 8 degrees Celsius)
- TE Buffer pH 8.0
- 10x Wash Buffer
- TWB Solution
- 2x NTB Solution
- LWB Solution
- NWB Solution
- Chromatin Capture Beads
- 10x Crosslink Reversal Buffer
- Strepavidin Beads
- 10x RBC Lysis Buffer
- 20% SDS
- 10% Triton X-100
- 100 mM MnCl2
- 0.5 M EDTA