*Only available in New Zealand
plexWell™ WGS 24 (WGS24) Highlights:
- plexWell NGS multiplexed library generation kit for Illumina®
- Assay-ready in 96-well fully-skirted low-profile PCR plate
- 24 unique barcode combinations
Description:
- Library prep for 24 gDNA samples for sequencing on the NovaSeq 6000
- 24-plex for higher-complexity human/plant/animal gDNA library prep
- Uniform >500bp insert sizing and better read balance across samples
- Kit contains all major reagents necessary to prepare Illumina-compatible NGS libraries including MagwiseTM paramagnetic beads (DNA polymerase not included)
Recommended Application:
High-complexity genome sequencing
- Human
- Animal
- Plant
plexWell™ WGS24 Library Prep Workflow:
plexWell™ WGS24 Library Prep Chemistry:
Specifications:
- Time-to-prepare a multiplexed library from 24 samples:
- Hands-on time: 1 hr
- Total elapsed time: 3 hrs
- DNA Input: 200 ng of genomic DNA per sample (n=24)
- Expected Results:
- Library Yield: 750 – 1500 fmoles of purified multiplexed library
- Library Diversity: <6 % dupe rate when human genomic DNA is sequenced to 30X depth
- Substantial normalization: Typically <1.5-fold range (min to max) in read count across all 24 sample
- Compatibility with Illumina sequencing systems:
- Optimized for sequencing performance on the NovaSeq 6000 in cooperation with Illumina
- Also compatible with Illumina MiSeq®, NextSeq®, HiSeq® instrument platforms
plexWell™ WGS 24 Resources