Synthetic sgRNA Kit
High-efficiency guide RNAs for knockouts and knock-ins.
CRISPR experts agree that using synthetic single guide RNAs (sgRNAs) for genome engineering generate more efficient and consistent editing results than any other type of guide RNAs.
Synthego sgRNAs are highly pure guides that deliver indel frequencies of up to 99%, making it easy to efficiently conduct knockout or knock-ins in virtually any cell type. Their sgRNAs can be ordered with site-specific chemical modifications that provide greater stability and decreased activation of the cellular innate immune response, resulting in superior editing in essentially every experiment.
Powered by Synthego’s proprietary Halo platform, sgRNA is available at any scale 1.5 nmol to 500 nmol.
Are you developing a CRISPR-based cell or gene therapy? Learn about Synthego’s large scale RUO and GMP-grade capabilities.
CRISPR Success is Within Your Reach
Synthego Delivers Robust and Reliable CRISPR Editing
Chemically modified synthetic sgRNAs from Synthego result in consistently high indel frequencies that enable robust editing. If you are using plasmids, you are not getting the results you deserve.
Synthego sgRNA Outperforms cr:tracrRNA >95% of the Time
Chemically modified synthetic sgRNA from Synthego produce higher indel frequencies than 2-part cr:tracrRNA guides in nearly every instance.
The most reliable knockout strategy for any human or mouse-derived cell lines.
Human and mmouse knockouts have never been easier. Common CRISPR knockout strategies rely on an individual guide RNA that produces random indels. This strategy is inefficient due to the wide variety and unpredictability of edits that can occur and often does not generate a functional knockout.
The Gene Knockout Kit v2 utilizes a proprietary multi-guide strategy that consists of up to three spatially coordinated modified sgRNAs that induce fragment deletion. With bioinformatic design powered by Synthego’s Halo platform, Synthego’s design tool generates a custom multi-guide sgRNA that reliably knocks out any human or mouse protein-coding gene on the first try.
Choose your gene of interest, and Synthego will synthesize your high-quality multi-guide sgRNA kit! If you don’t achieve knockout at the protein level, you will get your money back (human kit only).
Each kit arrives with one tube of target-specific multi-guide sgRNA (up to 3 sgRNAs/tube). The multi-guide sgRNA is chemically modified to resist degradation and prevent triggering intracellular immune responses. For best results, add the Transfection Optimization Kit to optimize for your cell type.
Novel Multi-guide Design for Protein-level Knockout
Powered by a unique multi-guide algorithm, the Gene Knockout Kit v2 enables knockout of any human or mouse protein-coding gene. To help you optimize your experiment, a Transfection Optimization Kit that includes a control multi-guide sgRNA and Cas9 (human), or a Rosa26 control sgRNA (mouse) are available.
Human or mouse CRISPR knockout libraries for confident target discovery.
Transform your target discovery with Synthego sgRNA Libraries. Choosing a reliable screening strategy is crucial in early drug discovery. Synthego’s Halo platform enables the design and synthesis of CRISPR arrayed libraries, reshaping target discovery to be more reliable than ever, mitigating false hits and alleviating data deconvolution.
Synthego’s Standard and Custom Arrayed CRISPR Screening Libraries comprise sgRNAs targeting one gene per well in a multiwell plate format. The guide sequences are designed and based on a proprietary multi-guide RNA strategy whereby up to three synthetic sgRNAs jointly create large fragment deletion in the target gene, resulting in a functional knockout. With this multi-guide strategy, arrayed approach, and high-quality synthetic sgRNA, you can reliably link genotype to phenotype using a wide variety of phenotypic assays uncover hits with confidence.
Synthego offers 30+ pre-designed standard libraries including the whole human, druggable, GPCRs, kinases, and immuno-oncology targets. These libraries are well-suited for target identification studies, providing a comprehensive gene set to begin your discovery process.
Synthego also offers custom libraries so you can choose your specific gene set from human and mouse genomes, well-suited for target validation studies. Custom libraries are shipped in as quickly as one week. All libraries arrive ready-to-transfect, eliminating laborious and time-intensive steps that alternative pooled libraries require. Synthego’s high-quality sgRNA libraries can be used to screen any human or mouse cell type, including primary cells.
Upgrade Your CRISPR Library. Discover the New Way to Screen
Unlike other screening strategies that result in insufficient knockouts and non-specific targeting, Synthego’s synthetic sgRNA libraries provide many distinct advantages by targeting one gene per well across a multiwell plate.
Options to Suit Your Workflow
Standard and custom libraries are available in a variety of formats to accommodate your throughput and equipment.
| Standard Libraries | Custom Libraries | |
|---|---|---|
| Species | Human | Human Mouse |
| Sizes Available | 30 pmpl per well* 150 pmol per well | 1.5 nmol per well (96-well) 150 pmol per well** (384-well) 250 pmol per well (384-well) |
| Well Format | 384-well plate | 96-well plate 384-well plate |
| Plate Format | Standard propylene Echo® qualified microplates | Standard propylene (96- or 384-well) Echo® qualified microplates (384-well) |
Synthego offers a range of sgRNA dispense amounts, plate well formats, in addition to Echo qualified microplates to fit your standard of custom library workflow. Libraries are well-suited for target identification and target validation studies.
*Only available for Whole Human Genome or Complete Duggable Libraries.
**150 pmol also available in Echo® qualified 384-well plates
Advanced RNA
Redefining What’s Possible with World-Leading RNA Chemistry and Synthesis Innovations.
RNA Unique to Your Needs.
You’re doing advanced research so you shouldn’t settle for standard RNA. Synthego’s Advanced RNA can be tailored to your specific research needs, delivering high-quality fully bespoke synthetic RNA molecules.
Synthego can synthesize guide RNAs that are compatible with any CRISPR nuclease, including all engineered versions of SpCas9, Cas12a (Cpf1), SaCas9 and Cas13a. They can also alter the secondary structure of the guide RNAs to your requirements.
Tell us what you need and we will deliver a high-quality customized solution, at any production scale, even if you have previously been told it cannot be made.
Advanced RNA Synthesis to Power Your Innovations.
You don’t run one-size-fits-all experiments, so you need Advanced RNA to meet your unique needs. Synthego’s proprietary RNA synthesis platform delivers flexibility in RNA length and modifications at the quality needed to achieve the best results. Synthetic RNA is available at the production scale you need, from small-scale testing, through preclinical development, all the way through clinical trials.
High Purity Synthetic RNA
Synthego understand you have complicated RNA needs. Their synthetic RNA chemistry is not length-limited, removing many limitations you may have previously experienced with other methods.
Synthetic sgRNA GMP Manufacturing for CRISPR-based Therapeutics and Diagnostics.
Synthego is the genome engineering leader. They have applied their expertise in CRISPR genome editing to a state-of-the-art Good Manufacturing Practice (GMP) production facility to produce GMP-grade synthetic single guide RNA (sgRNA). Synthego can take you through early-phase research, process development, and into the clinic with their new sgRNA GMP manufacturing capability.
Synthego’s GMP facility operates a quality manufacturing system that meets ICH Q7 Good Manufacturing Practice Guidance for Active Pharmaceutical Ingredients, Section 19, APIs for use in clinical trials.
From research to clinical application, Synthego can support the development of cell and gene therapies.
From Early-Phase Research to the Clinic
Synthego offers a range of custom sgRNA manufacturing solutions for every stage of your CRISPR-based research, therapeutic development, or diagnostic tool studies. Their facility can manufacture sgRNA according to your purification specifications so you receive material that meets your exact project needs within your research phase. Whether you require small or large scale research-grade, an intermediate grade “GMP-like”, or full GMP-grade sgRNA, Synthego can provide you a custom solution. All sgRNA manufacturing processes are provided with the appropriate documentation to support your project as you prepare for IND submission.
Synthego’s sgRNA team and facility are proud to offer you ample manufacturing capacity, validated processes, and proven record of CRISPR-editing expertise as you successfully progress toward clinical development.
Synthego Offers Custom sgRNA Manufacturing Options
Scaling up your sgRNA has never been easier. Synthego offer a range of sgRNA manufacturing options to suit your project need, from large scale RUO, an intermediate GMP-like, to fully certified GMP sgRNA material.